人間のシス調節要素と転写因子結合部位の哺乳類の進化
Gregory Andrews1, Kaili Fan1, Henry E Pratt1
1Program in Bioinformatics and Integrative Biology, University of Massachusetts Chan Medical School, Worcester, MA, USA.
Science (New York, N.Y.)
|April 27, 2023
まとめ
研究者は人間の 制御要素の進化の経路をマップし 何千もの制限を特定しました 欠かせない遺伝子の近くにある 制限された要素は 基本的な細胞の役割を強調し 霊長類特有のものは 免疫のような環境の相互作用と結びついています
科学分野:
- ゲノミクス
- 進化生物学
- バイオ情報学
背景:
- ヒトゲノムの規制の枠組みを理解することは 現代の生物学にとって極めて重要です
- 候補シス調節要素 (cCREs) と転写因子結合部位 (TFBS) は遺伝子調節の鍵となる.
研究 の 目的:
- 哺乳類のゲノム全体でヒトのcCREsとTFBSの進化軌道を図表する.
- 進化的制約下での規制要素とその関連遺伝子機能を特定する.
- 転置可能な要素から派生したTFBSの起源と進化を調査する.
主な方法:
- 241個の哺乳類のゲノムを 参照なしで並べた
- ヒトのcCRE090万個とヒトのTFBS1560万個を分析した.
- 制限された要素の識別と配列変数のための濃縮分析.
主要な成果:
- 439,461のcCREsと2,024,062のTFBSが進化的制約を受けていることが判明しました.
- 制限された要素に近い遺伝子は 基本的な細胞プロセスに関与しています
- 霊長類特有の要素に近い遺伝子は,嗅覚と免疫を含む環境相互作用と関連しています.
- 約20%のTFBSは,複雑な進化パターンのトランスポーザブル要素から派生しています.
- 複合的な特徴に関連した配列変数は,制限されたTFBSで強化されます.
結論:
- 進化的制約は,人間の規制要素の機能的意義を理解するための強力なレンズを提供します.
- この研究は,ヒトゲノムの規制機能とTFBSの進化動態を明らかにしています.
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