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関連する概念動画

Epigenetic Regulation01:37

Epigenetic Regulation

3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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CRISPR01:59

CRISPR

52.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.1K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

22.9K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

956
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
956
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

80
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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関連する実験動画

Updated: Jul 30, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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エピジェネティック・モディフィケーションをRNA機能とCRISPRベースの遺伝子調節のための反応的ハンドルとして利用

Qianqian Qi1, Xingyu Liu1, Fang Fu1

  • 1Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Sciences, The Institute of Molecular Medicine, Wuhan University People's Hospital, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan, Hubei 430072, China.

Journal of the American Chemical Society
|May 16, 2023
PubMed
まとめ

研究者は,5-ホルミルシチジン (f5C) 操作を用いた新しいRNA制御戦略を開発した. この方法はRNAの折りたたみ,結合,酵素の相互作用を制御し,CRISPR遺伝子調節の有望性を示しています.

さらに関連する動画

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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関連する実験動画

Last Updated: Jul 30, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

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Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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科学分野:

  • 生物化学
  • 分子生物学
  • 合成生物学

背景:

  • RNAの機能を in vivoで制御する現在の方法は限られている.
  • 精密なRNA操作のための新しい戦略の開発は,生物学的研究と治療上の応用にとって極めて重要です.

研究 の 目的:

  • 5-ホルミルシチジン (f5C) 誘導塩基操作を用いたRNA機能の制御のための新しい戦略を導入する.
  • RNAの折りたたみ,小分子結合,酵素認識を調節する小分子の能力を実証する.
  • この戦略をクラスタ化された定期間隔の短いパリンドロミック・リピート (CRISPR) システムに適用することを検討する.

主な方法:

  • f5C指向の塩基操作のために,マロニトリルとピリジンボランを使用した.
  • これらの反応剤がRNAの折り畳み,小分子結合,酵素認識に及ぼす影響を調査した.
  • 2つの異なるCRISPRシステムを制御するためにf5C指向反応を適用しました.

主要な成果:

  • マロノニトリルおよびピリジンボランは,f5Cを含むRNAの折り畳み,小分子結合,および酵素認識を効果的に操作した.
  • f5C指向反応を用いた2つの異なるCRISPRシステムの成功制御が実証された.
  • 小分子ベースのRNA制御の可能性を検証した.

結論:

  • f5C指向の塩基操作戦略は,RNA機能を制御するための新しいアプローチを提供します.
  • この方法はCRISPRベースの遺伝子発現と他の生物学的応用を制御する可能性を示している.
  • さらに in vivo の最適化が必要ですが,小分子ベースのアプローチは重要な機会を提供します.