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関連する概念動画

Types of RNA01:23

Types of RNA

64.1K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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RNA Interference01:23

RNA Interference

26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.9K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.9K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.7K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
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Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

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非活性RNA結合小分子を生物活性分解剤にプログラムする

Yuquan Tong1, Yeongju Lee1, Xiaohui Liu1

  • 1Department of Chemistry, The Scripps Research Institute & The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Jupiter, FL, USA.

Nature
|May 24, 2023
PubMed
まとめ
この要約は機械生成です。

研究者たちは小さな分子が RNA とどのように相互作用するかを調査し,多くのものが弱い結合をしていることを発見しました. 彼らはこれらの結合物質を使用して標的RNAを分解する新しい方法を開発し,病気に関連するマイクロRNA-155とmRNAを成功裏に標的とした.

さらに関連する動画

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

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関連する実験動画

Last Updated: Jul 29, 2025

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

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科学分野:

  • 分子生物学
  • 薬剤化学
  • RNAセラピー

背景:

  • RNAへの小さな分子結合は,生物学的活動を達成するために困難です.
  • RNAと小さな分子の相互作用を理解することは 薬の発見に不可欠です

研究 の 目的:

  • 小分子と3DRNA構造の間の分子認識パターンを調査する.
  • 小さな分子を用いてRNA生物学を調節する戦略を探求する.
  • 治療用途のRNA標的分解剤を開発する.

主な方法:

  • 自然製品にインスパイアされた小分子集合と折りたたまれたRNA構造との相互作用を研究した.
  • ヒトのトランスクリプトーム全体でRNA-小分子相互作用の地図を作成した.
  • リボヌクレアスを標的とするキメラを設計し,RNAの分裂を誘導する.
  • 先駆体マイクロRNA-155,JUN mRNA,MYC mRNAに対する検証された分解剤.

主要な成果:

  • RNA-小分子相互作用の構造-活性関係を特定した.
  • 結合場所により,ほとんどの相互作用は生物学的に無効であることが予測された.
  • 不活性な結合物質が強力なRNA分解剤に変換できることを示した.
  • 特定のRNAターゲットを対象に 選択的な分解剤を成功裏に設計した.

結論:

  • 小分子-RNAの相互作用は,標的型RNAの分解のために利用できます.
  • リボヌクレアスを標的とするキメラは,弱く結合する物質を強力なRNA機能の調節剤に変換する戦略を提供します.
  • このアプローチはRNAを標的とする新種の治療法の開発に 有望である.