関連する実験動画

Updated: Aug 31, 2026

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

病気の理解のためにNMRスペクトロスコピーの使用

G K Radda

    Science (New York, N.Y.)
    |August 8, 1986
    PubMed
    まとめ

    核磁共振スペクトロスコーピーは,人間の組織のバイオエネルギー学を非侵襲的に評価します. この技術は,筋肉疾患と全身機能の理解を向上させ,生化学と臨床実践を橋渡しする.

    さらに関連する動画

    Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins12:47

    Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

    Published on: December 27, 2016

    Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics11:02

    Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

    Published on: November 29, 2024

    関連する概念動画

    Nuclear Magnetic Resonance (NMR): Overview01:07

    Nuclear Magnetic Resonance (NMR): Overview

    Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
    NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

    ¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

    When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
    Two-Dimensional (2D) NMR: Overview01:12

    Two-Dimensional (2D) NMR: Overview

    The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
    The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
    Applications Of NMR In Biology01:25

    Applications Of NMR In Biology

    Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
    The...
    Double Resonance Techniques: Overview01:12

    Double Resonance Techniques: Overview

    Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
    Spin decoupling is usually achieved by...
    2D NMR: Overview of Heteronuclear Correlation Techniques01:18

    2D NMR: Overview of Heteronuclear Correlation Techniques

    Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.