細胞外シトクロームナノワイヤは,プロカリオットに普遍的に存在しているようです
Diana P Baquero1, Virginija Cvirkaite-Krupovic1, Shengen Shawn Hu2
1Institut Pasteur, Université Paris Cité, CNRS UMR6047, Archaeal Virology Unit, Paris 75015, France.
Cell
|June 8, 2023
まとめ
細胞外サイトクロームナノワイヤ (ECN) は,現在,細菌だけでなく,古生物にも存在しています. 電子の伝達に不可欠な 伝導性タンパク質フィラメントは 広範な生物学的メカニズムであるようです
科学分野:
- 微生物学
- 生物化学
- 構造生物学
背景:
- Geobacter sulfurreducensの細胞外サイトクロムナノワイヤ (ECN) と特定された電気伝導付属体は,潜在的な応用のために重要な関心を得ています.
- 細菌以外の生物における電子移転のための同様のECNの流行は,ほとんど知られていなかった.
研究 の 目的:
- 細胞外シトクロームナノワイヤ (ECN) の存在と構造を考察する.
- ECNが生命の異なる領域に 電子を転送する広範なメカニズムであるかどうかを判断する.
主な方法:
- 低温電子顕微鏡を用いて,高熱性アーカイアからのECNの原子構造を決定した.
- 生物情報分析は,様々なアーカイア系およびプラズミドのECNの同類を特定するために使用されました.
主要な成果:
- 超熱性アーカイアからの2つの異なるECNの原子構造が解明された.
- Archaeoglobus veneficus ECNの同種は,メソフィルのメタン酸化およびアルカン分解アーカイア,およびボルグメガプラズミドで発見されました.
- タンパク質の折りたたみが異なるにもかかわらず,特定されたECNは保存されたヘム配列を共有し,電子移転のための最適化されたパッキングを示しています.
結論:
- 古代生物におけるECNの発見は,これらの導電性タンパク質フィラメントが,遠距離電子伝送の共通かつ広範なメカニズムであることを示唆している.
- この発見は,細胞外電子伝達の既知の生物学的な役割を,細菌と古生物の両方のドメインに拡張します.
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