関連する実験動画
Updated: Jul 25, 2025
08:55
Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
Published on: August 7, 2018
10.9K
AMPK媒介によるRIPK1のリン酸化による細胞死亡の代謝オーケストレーション
Tao Zhang1, Daichao Xu2,3, Elijah Trefts4
1Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Science (New York, N.Y.)
|June 29, 2023
まとめ
アデノシンモノフォスファート活性化タンパク質キナーゼ (AMPK) は,RIPK1を阻害することで,エネルギーストレス中に細胞死亡を防ぐ. この代謝チェックポイントは 細胞の運命,代謝,炎症を統合します
科学分野:
- 細胞生物学
- メタボリズム
- 分子生物学
背景:
- アデノシンモノフォスファート活性化タンパク質キナーゼ (AMPK) は,エネルギーストレスの代謝適応を調節する.
- 持続的な代謝ストレスは細胞死につながるが,この過程におけるAMPKの役割は不明である.
- 受容体相互作用タンパク質キナーゼ1 (RIPK1) の活性化は,ストレス誘発の細胞死に関与している.
研究 の 目的:
- AMPKが代謝ストレス下での細胞死に影響を与えるメカニズムを解明する.
- AMPK,RIPK1と細胞運命を決定する相互作用を調査する.
- 代謝ストレスに関連する疾患の潜在的治療標的を特定する.
主な方法:
- 代謝ストレスへの反応としてAMPKとRIPK1の活性化を研究した.
- 機能的影響を評価するために遺伝子操作 (Ampk欠乏症,RIPK1 S415A変異) を利用した.
- in vivoの関連性を評価するために,不血性損傷のマウスモデルを使用した.
主要な成果:
- TRAIL受容体を通してRIPK1を活性化する.
- AMPKはRIPK1の活性化を阻害し,Ser415でリン酸化し,それによって細胞死亡を抑制した.
- Ampk欠乏またはRIPK1 S415A変異はRIPK1の活性化と細胞死を促進しました.
- Ampkα1 欠乏したマウスの血性損傷から保護された RIPK1 の遺伝的不活性化.
結論:
- RIPK1のAMPKリン酸化は,ストレス下での細胞運命を制御する重要な代謝チェックポイントです.
- AMPK-RIPK1軸は代謝,細胞死,炎症を統合する.
- この軸は,代謝ストレスと細胞死を含む状態のための新しい治療目標を表しています.
さらに関連する動画
06:12Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum
Published on: May 3, 2024
2.1K
09:15Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
Published on: October 20, 2022
2.3K
関連する概念動画
01:22PI3K/mTOR/AKT Signaling Pathway
3.7K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.7K
01:18Autophagic Cell Death
3.5K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.5K
01:16Necrosis
4.6K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.6K
01:30Overview of Cell Death
7.4K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.4K
01:17The Extrinsic Apoptotic Pathway
6.5K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.5K
01:31The Intrinsic Apoptotic Pathway
6.6K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K