Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

16.4K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
16.4K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.3K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.3K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.5K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
Exon Recombination02:32

Exon Recombination

3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.6K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

m<sup>6</sup>A reader Ythdf proteins control retrotransposon B2 repeat expression and safeguard early embryo development.

The EMBO journal·2026
Same author

CircTarget: a comprehensive database of circRNA-target RNA interactions across multiple cell types.

Nucleic acids research·2025
Same author

ssG4-seq for global profiling of strand-specific G-quadruplex structures in mammalian genomes.

Nature communications·2025
Same author

Mechanism of DNA targeting by human LINE-1.

Science (New York, N.Y.)·2025
Same author

Identification and characterization of human retinal stem cells capable of retinal regeneration.

Science translational medicine·2025
Same author

The nuclear exosome co-factor MTR4 shapes the transcriptome for meiotic initiation.

Nature communications·2025

関連する実験動画

Updated: Jul 23, 2025

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.2K

補完的な Alu 配列は強化剤-促進剤の選択性を媒介する.

Liang Liang1, Changchang Cao1, Lei Ji1

  • 1Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Nature
|July 12, 2023
PubMed
まとめ
この要約は機械生成です。

Alu要素は,補完的なRNA相互作用を通じてエンハンサー・プロモーターのループを媒介する. この発見は遺伝子調節のメカニズムを明らかにし,がんのリスクを含む分子機能にノンコーディングの変異をリンクしています.

さらに関連する動画

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

2.7K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.4K

関連する実験動画

Last Updated: Jul 23, 2025

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.2K
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

2.7K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.4K

科学分野:

  • ゲノミクス
  • 分子生物学
  • エピジェネティクス

背景:

  • 強化剤は,プロモーターと相互作用することで遺伝子発現を調節する.
  • 強化剤が同類のプロモーターを見つけるメカニズムは,ほとんど不明である.

研究 の 目的:

  • 増強剤-促進剤の接続性におけるRNAの相互作用の役割を調査する.
  • 増強剤-プロモーターRNAの相互作用をマッピングし,関連するゲノム要素を特定する.
  • 遺伝子変異を分子機能と結びつけるため

主な方法:

  • RNA in situ 適合配列解析 (RICS) 技術は,増強剤-促進剤 RNA 相互作用マップを生成するために適用されました.
  • 強化剤と促進剤のRNA相互作用部位との重なり合いの分析
  • Alu要素のノックアウトとテザリングに関する機能実験.
  • 増強剤と促進剤の相互作用のマップに非コーディングリスクの変数をマップする.

主要な成果:

  • 増強剤- プロモーターRNAの相互作用部位の37. 9%がAlu配列と重複した.
  • Aluと非- AluのRNA配列は互補性を示し,複合形成を示唆した.
  • Alu要素の操作 (ノックアウト,挿入,テザリング) は強化剤-促進剤のループに影響を及ぼした.
  • 非コード変異を遺伝子機能と結びつけるための枠組みが確立され,タンパク質をコードする遺伝子に影響を与えるAlu要素内の何千もの変異を特定しました.
  • PTK2増強剤への多形アルウの挿入は腫瘍発生と関連していた.

結論:

  • Aluエレメントは,RNA複合形成を通じてエンハンサー・プロモーターのペアリング特異性を媒介する上で重要な役割を果たします.
  • この研究は,非暗号化リスク変数の分子機能を解釈する方法を提供します.
  • この発見は遺伝子調節と 病気への影響について 新たな理解をもたらします