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関連する概念動画

G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

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GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
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Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

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Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
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Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

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Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several...
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Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

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Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
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Ion Channels01:19

Ion Channels

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The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
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Non-gated Ion Channels01:24

Non-gated Ion Channels

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Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
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Updated: Jul 20, 2025

Harvesting Venom Toxins from Assassin Bugs and Other Heteropteran Insects
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ヒトのSTINGは 陽子チャンネルです

Bingxu Liu1,2,3, Rebecca J Carlson1,4, Ivan S Pires3

  • 1Broad Institute, Cambridge, MA, USA.

Science (New York, N.Y.)
|August 3, 2023
PubMed
まとめ

インターフェロン遺伝子の刺激剤 (STING) は,細胞プロセスを調節するプロトンチャネルとして作用する. このチャネル活動は,炎症体活性化とLC3B脂質化に不可欠ですが,インターフェロンの誘導には不可欠ではありません.

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Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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科学分野:

  • 免疫学
  • 細胞生物学
  • 分子生物学

背景:

  • 臓器細胞からの陽子漏れは,非正規の軽鎖3B (LC3B) の脂化と炎症体の活性化を信号する.
  • これらのプロセスは通常,インターフェロン遺伝子の刺激器 (STING) の活性化によって誘発されます.

研究 の 目的:

  • ヒトのSTINGが 陽子チャンネルとして機能するという仮説を 調べるためだ
  • LC3Bの脂化と炎症体の活性化のような下流の細胞イベントにおけるSTINGの潜在的なチャネル活動の役割を決定する.

主な方法:

  • STINGの推定チャネルインターフェイスを特定するための構造分析.
  • STINGの活性化時にゴルギのpH変化を測定する.
  • リポソームでSTINGを再構成して 陽子輸送を評価する
  • STING活性と陽子の流れを抑制するために,STINGアゴニストである化合物53 (C53) を利用する.
  • STING誘発のLC3B脂化と炎症体の活性化に対するC53の影響を評価する.

主要な成果:

  • スティングの活性化により ゴルギのpHが上昇し 陽子の流出が示されました
  • 再構成されたSTINGはリポソームに 陽子輸送能力を示した.
  • 化合物53 (C53) は,細胞モデルとリポソームの両方で,STING誘発の陽子流を効果的に阻害しました.
  • C53はまた,STING誘発のLC3B脂化と炎症体の活性化を阻害し,これらの経路に対するSTINGのチャネル機能の必要性を強調した.

結論:

  • ヒトのSTINGは 陽子チャンネルとして機能する
  • STINGの陽子チャネル活動は,LC3Bの脂化と炎症体の活性化を誘発するために不可欠です.
  • STINGのインターフェロン誘導機能は,LC3B脂化と炎症体の活性化における役割から分離され,異なる機能的メカニズムを示唆する.