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免疫細胞アトラスは,胎児の発達中の人のマクロファージの仕様の動態を明らかにしています.

Zeshuai Wang ¹, Zhisheng Wu ², Hao Wang ³

¹Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
²Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; School of Chemistry and Chemical Engineering, Southeast University, Nanjing, China.
³Maternal Fetal Medicine Institute, Department of Obstetrics and Gynaecology, Shenzhen Baoan Women's and Children's Hospital, Jinan University, Shenzhen, China.
⁴Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
⁵Shanghai Key Laboratory of Metabolic Remodeling and Health, Institute of Metabolism and Integrative Biology, Centre for Evolutionary Biology, Fudan University, Shanghai, China; Shanghai Qi Zhi Institute, Shanghai, China.
⁶State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China.
⁷Department of Systems Immunology, Weizmann Institute of Science, Rehovot, Israel.
⁸Department of Gynaecology & Obstetrics, Shenzhen University General Hospital, Shenzhen University, Shenzhen, China.
⁹Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China.
¹⁰Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Maternal Fetal Medicine Institute, Department of Obstetrics and Gynaecology, Shenzhen Baoan Women's and Children's Hospital, Jinan University, Shenzhen, China.
¹¹Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; College of Biological Sciences, China Agricultural University, Beijing, China.
¹²The Brain Cognition and Brain Disease Institute, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
¹³Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Graduate School of Peking Union Medical College, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
¹⁴University of Chinese Academy of Sciences Shenzhen Hospital, Shenzhen, China.
¹⁵Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China.
¹⁶College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
¹⁷Jinxin Fertility Group Limited, Chengdu, China.
¹⁸Shenzhen Key Laboratory for Reproductive Immunology of Peri-Implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.
¹⁹School of Chemistry and Chemical Engineering, Southeast University, Nanjing, China; Department of Immunology, Nanjing Medical University, Nanjing, China.
²⁰Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; INSERM U1015, Gustave Roussy Cancer Campus, Villejuif 94800, France; Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A(∗)STAR), 8A Biomedical Grove, Immunos, Singapore 138648, Singapore; Translational Immunology Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore 169856, Singapore.

⁰Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.

Cell +

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2023年9月13日

PubMedで要約を見る

まとめ

人間のマクロファージは多様で発達に不可欠ですこの研究では,胎児の発達中のサブタイプと機能をマッピングし,様々な組織におけるマイクログリアのような細胞とメラノサイトの発達におけるその役割を明らかにしています.

科学分野

発達生物学
免疫学
単細胞ゲノム

背景

マクロファージは健康や病気に多様な役割を果たす重要な免疫細胞です
ヒトにおけるマクロファージの異質性と発達の理解は限られている.
ヒトの発達過程における正確な機能と起源については,さらなる解明が必要である.

研究の目的

妊娠初期から胎児期の末期までの人間のマクロファージの発達に関する包括的な単細胞マップを作成する.
異なるマクロファージのサブタイプとその機能的役割を特定し,特徴づけること.
人間の胎児のマクロファージの起源と動態を調査する.

主な方法

19人の胎児組織 (PCW 4-26) の単細胞RNAシーケンシング (scRNA-seq).
空間と分化軌道の分析
特定されたマクロファージ集団の分子および形態学的特徴.

主要な成果

15種類のヒトマクロファージのサブタイプを特定しました
胎児の表皮,精巣,心臓に微小状の集団が発見され, 神経頂部細胞と相互作用している.
胎児の臓器の周周で発見されたプロ血管性マクロファージの特徴.
胎児の表皮の初期の免疫細胞としてマイクログリアのような細胞の実証.

結論

ヒトのマクロファージは胎児発達の過程で有意な異質性を示す.
マイクログリアのような細胞は,神経の細胞分化をメラノサイトに調節する役割を果たします.
血管新生性マクロファージは卵黄から派生し,血管の発達に寄与する.
この研究は,ヒトの胎児マクロファージの生物学と機能の基礎的なアトラスを提供しています.

キーワード:

血管新生発達免疫学ヒト免疫学免疫細胞アトラスマクロファージマイクログリアマイクログリアのような細胞ニューラル・クライスト細胞周周マクロファージ scRNA-seq について

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