関連する実験動画

Updated: Jul 15, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

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Published on: November 1, 2024

678

AsCas12fベースのコンパクトゲノム編集ツール,深層変異スキャニングと構造分析によって得られた

Tomohiro Hino1, Satoshi N Omura2, Ryoya Nakagawa2

  • 1Department of Cardiovascular Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.

Cell
|September 30, 2023
PubMed
まとめ

エンジニアリングされたコンパクトのCas12f変種 (enAsCas12f) は,ヒト細胞とマウスのゲノム編集が強化されていることを示しています. これらの変種はアデノ関連ウイルス (AAV) 伝達に適しており,体内の遺伝子治療のための最小限のプラットフォームを提供します.

キーワード:
CRISPR-Casについて動物実験クリオ・エム深層変異スキャニング遺伝子療法ゲノム編集iPS セル

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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