まとめ
インヴァリアントチェーン (In) タンパク質は,単一のmRNA上の2つのAUG開始部位の代替使用を通じて,p33とp35の2つの形態で生成されます. 人間の細胞で観察されたこのメカニズムは,この種の最初の非ウイルスの例です.
科学分野:
- 分子生物学は分子生物学である.
- 免疫学 免疫学とは
- タンパク質合成 タンパク質合成
背景:
- インヴァリアントチェーン (In) は,Ia抗原と関連しています.
- 人間では,Inは主に2つの関連形態であるp33とp35として存在します.
研究 の 目的:
- 2つの異なるインタンパク質形態 (p33およびp35) の合成に責任のある分子機構を調査する.
- mRNAの代替翻訳開始部位が関与しているかどうかを判断する.
主な方法:
- 潜在的翻訳開始部位 (AUG) のmRNA配列の分析.
- 特定のAUGが欠けている改変されたcDNAクローンを持つ細胞の変異.
- 合成mRNAを用いた細胞フリートランスレーションアッセイをインビトロで行いました.
主要な成果:
- インヴァリアントチェーンmRNAには2つのインフェーズAUGスタートコドンが含まれています.
- 完全長さのcDNAはp33とp35の両方を生成します.
- 最初のAUGを削除すると,p33形式のみが表示されます.
- 2番目のAUGの変異は,p35形のみを生成する.
結論:
- 単一のmRNA上の2つのインフェーズAUGでの代替イニシエーションは,p33およびp35不変連鎖タンパク質の合成を決定する.
- これは,非ウイルスのシステムにおけるこの翻訳制御メカニズムの最初の文書化された例を表しています.
関連する概念動画
Antibody Structure
51.8K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
51.8K
Antigens Involved in Adaptive Immunity
1.7K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
1.7K
Diversity of Antigen Receptors
2.1K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
2.1K
Antigen Processing Pathways
2.9K
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
2.9K
T Cell Activation and Clonal Selection
13.7K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
13.7K
B Cell Activation and Differentiation
14.4K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
14.4K


