関連する実験動画
Updated: Jul 13, 2025

11:14
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
Published on: December 10, 2021
1.6K
Wnt生物生成,分泌,およびWnt7特有のシグナリングの分子基礎
Xiaofeng Qi1, Qinli Hu1, Nadia Elghobashi-Meinhardt2
1Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Cell
|October 18, 2023
まとめ
カルレチクリン (CALR) はWntタンパク質の生体生成に伴い,Porcupine (PORCN) からWntless (WLS) への移行を促進します. Wnt-bound WLSの脂質はWnt分泌を制御し,RECKはWnt7シグナリングを活性化する.
科学分野:
- 分子生物学
- 細胞生物学
- 生物化学
背景:
- Wntタンパク質は,Porcupine (PORCN) によって脂化され,Wntless (WLS) に結合して分泌される.
- ERからのWnt輸送のメカニズムは,まだ完全に理解されていません.
- Wnt7aは中枢神経の血管新生と血脳障壁の整合性にとって不可欠です.
研究 の 目的:
- Wntの生体生成とWnt7特有のシグナル伝達原理を明らかにする.
- Wnt-WLS複合体の形成におけるカルレチクリン (CALR) の役割を決定する.
- Wntの分泌とWnt7-RECKの相互作用のメカニズムを調査する.
主な方法:
- Wnt7a-WLSとWnt7a-RECK複合体の構造分析
- Wntのバイオゲネシスとシグナリングを評価する機能的測定法
- Wnt-WLSの相互作用を研究するための分子動力学シミュレーション
主要な成果:
- カルレチクリン (CALR) はWnt7a- WLS複合体と結合し,チャペロンとして作用する.
- Wnt-bound WLS内のフォスフォリピドは,Wntの結合と解離を調節する.
- 構造的な洞察は,RECKがN端領域を通じてWnt7固有のシグナリングを活性化する方法を示しています.
結論:
- CALRは,Wntの生殖過程で,WntをPORCNからWLSに転送することを促進する.
- 脂質媒介メカニズムはWntの分泌を制御する.
- Wnt7a-RECK構造は,Wnt7特有のシグナリングアクティベーションを明確にします.
関連する概念動画
Canonical Wnt Signaling Pathway
8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Notch Signaling Pathway
4.3K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Catenins
2.3K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.3K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K

