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RNA Stability01:53

RNA Stability

33.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Overview of DNA Repair02:25

Overview of DNA Repair

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In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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関連する実験動画

Updated: Jul 12, 2025

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
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Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

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治療目的のオリゴヌクレオチドを設計するためのRNA/DNAハイブリッド複合体の細胞内安定性予測

Dipanwita Banerjee1, Hisae Tateishi-Karimata1, Maria Toplishek2

  • 1Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan.

Journal of the American Chemical Society
|October 24, 2023
PubMed
まとめ

細胞内の分子の混雑は RNA/DNAハイブリッド複合体を不安定にします 新しいパラメータは二重の安定性を予測し,遺伝子調節と核酸治療を助けます.

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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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関連する実験動画

Last Updated: Jul 12, 2025

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

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科学分野:

  • 分子生物学
  • バイオ物理学
  • 遺伝学

背景:

  • RNA/DNAハイブリッド・デュプレックスは細胞内の遺伝子発現を調節する.
  • 細胞の臓器細胞は 核酸構造に影響を与える 分子混雑を示します
  • この混雑がRNA/DNAハイブリッドに与える正確な影響は完全に理解されていません.

研究 の 目的:

  • RNA/DNAハイブリッドのデュプレックス安定性における分子混雑のメカニズム的詳細を調査する.
  • 様々な細胞条件下におけるハイブリッド・デュプレックス安定性の予測パラメータを開発する.
  • 生体細胞におけるこれらのパラメータの適用性を評価する.

主な方法:

  • 熱力学分析
  • 核磁共振 (NMR) スペクトロスコーピー
  • フォースター共鳴エネルギー転送 (FRET) 技術

主要な成果:

  • 分子混雑は,構造に依存した方法でRNA / DNAハイブリッド複合体を不安定化します.
  • purin - pyrimidine非対称性によって影響されるB型からA型へのハイブリッド二重変異は,水分と不安定化を増加させる.
  • 混雑した溶液と生きた細胞の両方に適用可能な予測パラメータを開発した.

結論:

  • 環境要因と二重構造が安定に与える量的な貢献
  • パラメータは,多様な混雑条件でRNA/DNAハイブリッドの二重安定性を予測します.
  • 派生したパラメータは,遺伝子ターゲティング療法と診断の開発を導くことができます.