酵母タンパク質の相互作用体の社会的構造
André C Michaelis1, Andreas-David Brunner1,2, Maximilian Zwiebel1
1Max-Planck Institute of Biochemistry, Martinsried, Germany.
Nature
|November 15, 2023
まとめ
研究者らはSaccharomyces cerevisiaeの相互作用をマッピングし,何千ものタンパク質相互作用を特定しました. この包括的な地図は 酵母タンパク質の高度な結合を明らかにし 細胞の機能を理解するのに役立ちます
科学分野:
- 分子生物学
- システム生物学
- プロテオミクス
背景:
- 細胞の機能は タンパク質とタンパク質の相互作用に依存し 生物学的システムを理解するのに不可欠です
- 現在のインタラクトームマップは,特に少量,膜,またはタグ感受性タンパク質複合体については不完全である.
- 既存のタンパク質相互作用データの信頼性の評価は困難です.
研究 の 目的:
- インタラクトームの総合的なマッピングのための敏感で高通量メソッドを開発する.
- 低濃度でタグ付けが難しいタンパク質を特定する際の限界を克服する.
- Saccharomyces cerevisiaeのほぼ飽和したインタラクトームマップを作成する.
主な方法:
- 質量スペクトロメトリー法と結合した高通量親和濃縮法を開発した.
- 量的な二次元分析戦略を用いて, 96 井戸の形式で容量を削減しました.
- 酵母タンパク質全体をカバーする内生GFPタグライブラリを分析した.
主要な成果:
- 4,159のプルダウンから 3,927のタンパク質と 31,004の相互作用の高度に構造化されたネットワークを生成しました.
- 既知のタンパク質の数を2倍にし 信頼性の高い相互作用を3倍にしました
- 非常に少量のエピジェネティックおよびオルガネル膜複合体を特定し,タグ付け不可能な複合体を推論した.
結論:
- イーストのインタラクタームは高度に相互接続されており,ほとんどのタンパク質は平均16のインタラクタを持っています.
- タンパク質の間の最短経路の平均値は 4.2 です 人間の社会的ネットワークと似ています
- AlphaFold-Multimerは,複合体内の非特徴化されたタンパク質に関する機能的な洞察を提供した.データはwww.yeast-interactome.orgで入手できます.
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