関連する実験動画

Updated: Aug 17, 2026

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

ブルーム症候群におけるDNAリガゼI欠乏症

A E Willis, T Lindahl

    Nature
    |January 22, 1987
    PubMed
    まとめ

    ブルーム症候群は珍しい遺伝疾患で,DNA修復の欠陥と関連している. この研究では,ヒトのDNAリガゼIの欠乏が,ブルーム症候群の細胞異常における重要な要因であると特定しています.

    さらに関連する動画

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence07:55

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence

    Published on: June 26, 2020

    Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence06:23

    Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

    Published on: January 17, 2025

    関連する概念動画

    Nucleotide Excision Repair01:08

    Nucleotide Excision Repair

    Overview
    Base Excision Repair01:54

    Base Excision Repair

    One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
    The first step of...
    Translesion DNA Polymerases02:10

    Translesion DNA Polymerases

    Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
    TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
    Fixing Double-strand Breaks02:04

    Fixing Double-strand Breaks

    The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
    Nucleotide Excision Repair01:38

    Nucleotide Excision Repair

    DNA Distortion and Damage
    Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
    Biosynthesis of Nucleic Acids01:28

    Biosynthesis of Nucleic Acids

    Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...