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関連する概念動画

Structural Protein Function01:56

Structural Protein Function

27.7K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
27.7K
Fibril-associated Collagen01:11

Fibril-associated Collagen

2.5K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
2.5K
Type IV Collagen of Basal Lamina01:05

Type IV Collagen of Basal Lamina

2.2K
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen  forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...
2.2K
Selectins01:25

Selectins

3.3K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.3K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

20.0K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
20.0K
Structure of Cadherins01:25

Structure of Cadherins

3.3K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
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関連する実験動画

Updated: Jul 6, 2025

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
07:54

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Published on: September 20, 2012

13.8K

コラーゲンヘテロトリマー

Valdrin Islami1, Philipp Bittner1, Tomas Fiala1

  • 1Laboratory of Organic Chemistry, ETH Zurich, D-CHAB, Vladimir-Prelog-Weg 3, 8093 Zurich, Switzerland.

Journal of the American Chemical Society
|December 29, 2023
PubMed
まとめ

合成コラーゲンヘテロトリマーを 設計した科学者は 特定の三重ヘリクセルに 自己組み立てをします このタンパク質アセンブリコードは 複数の鎖の分類を制御し 複雑なタンパク質構造を 精密に制御することを示しています

科学分野:

  • 生物化学
  • 材料科学
  • 合成生物学

背景:

  • 自然は,特にヘトロトリメリックコラーゲンのタンパク質組成を制御するために複雑なメカニズムを使用しています.
  • これらの自然なプロセスを理解し,複製することは,新しい生体材料と治療用タンパク質の開発に不可欠です.

研究 の 目的:

  • 合成コラーゲンヘトロトリマーの組成とレジスタ選択組成のための設計原則を確立する.
  • 特定のタンパク質構造の形成を制御するためのプログラム可能な"組み立てコード"を実証する.

主な方法:

  • コンプリメンタリー (4S) - アミノプロリンとアスパルテート残基を直接鎖相互作用に利用した.
  • 8つの異なるペプチド鎖を設計し 特定の配列で自己組織化を促進します
  • 組み立てられた構造を分析するために,ネイティブのエレクトロスプレーイオン化質量スペクトロメトリー (ネイティブESI-MS) を採用した.

主要な成果:

  • 合成コラーゲンヘトロトリマーの設計原理を確立しました.
  • 512の理論的組み合わせから8つの異なる糸を3つの特定のトリプルヘリックス構造に自己分類することを実証した.
  • ネイティブESI-MSは,共存するヘトロトリマーの正確で特異的な形成を確認した.

さらに関連する動画

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Production of Nanofibrillar Patterned Collagen for Tissue Engineering

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Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D
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Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D

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関連する実験動画

Last Updated: Jul 6, 2025

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen
07:54

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Published on: September 20, 2012

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Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

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Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D
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Revealing the Cytoskeletal Organization of Invasive Cancer Cells in 3D

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結論:

  • 開発された"組み立てコード"は,高特異性でタンパク質の自己組み立てをプログラムするための強力なツールを提供します.
  • この研究は,予測可能な結果を持つ,カスタムデザインされたタンパク質構造の作成の基礎を築きます.
  • 複雑な生物学的分子設計における合成生物学のアプローチの可能性を強調する.