関連する実験動画
Updated: Jul 6, 2025

08:46
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
Published on: June 16, 2022
2.5K
サイトネーム信号は,哺乳類の組織パターニングに不可欠な貢献をします
Eric T Hall1, Miriam E Dillard1, Elizabeth R Cleverdon1
1Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Cell
|January 3, 2024
まとめ
この研究では,ソニック・ヘッジホッグ (SHH) モルフォゲンは,特殊な細胞突起であるシトネームで運ばれていることが明らかになった. ミオシン10 (MYO10) はこれらのシトネームを調節し,胚の発達中に適切な組織パターンを確保します.
科学分野:
- 発達生物学
- 細胞生物学
- 神経科学
背景:
- モルフォゲン・グラデーションは 細胞の運命を指示することで 組織を形作ります
- 生体内での形態素輸送の直接的な視覚化が困難で,配送メカニズムが曖昧である.
研究 の 目的:
- 哺乳類の組織発達の過程における形態素輸送,特に音響ヘッジホッグ (SHH) のメカニズムを調査する.
- 細胞膜と関連するタンパク質のSHH発達の役割とその神経発達への影響を明らかにする.
主な方法:
- マウスモデルを開発した.
- 先進的な顕微鏡を用いた in vivo サイトネームの保存と視覚化のための最適化された方法.
- 細胞膜形成と機能におけるミオシン10 (MYO10) の機能を研究するために遺伝子操作を用いた.
主要な成果:
- 細胞内循環がシトネームへのSHHの負荷を容易にすることを示した.
- 開発中のマウスの神経管に内生性SHHの局所化を示した.
- 神経細胞の運命を変化させ 神経の発達を阻害する
- MYO10は細胞膜の長さと密度にとって重要で,SHHとWNTのシグナル伝達に影響する.
結論:
- サイトネームベースの輸送は,哺乳類の発達における形態素分散に不可欠である.
- MYO10は細胞系媒介信号の重要な調節体であり,適切な組織パターンと神経発達に不可欠です.
関連する概念動画
Microtubules in Signaling
1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
Cytoskeletal Coordination in Cell Migration
4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K
Notch Signaling Pathway
4.3K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.3K
Cadherins in Tissue Organization
3.0K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
3.0K
Hedgehog Signaling Pathway
7.4K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K
Cell Polarization by Rho Proteins
2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K

