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Updated: May 8, 2026

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
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Cu-Catalyzed Azide-Alkyne-Thiol Reaction Forms 化学プロテオミック研究における普遍的な背景について

Andreas Wiest1, Pavel Kielkowski1

  • 1Department of Chemistry, LMU Munich, Würmtalstr. 201, 81375 Munich, Germany.

Journal of the American Chemical Society
|January 12, 2024
PubMed
まとめ

銅で触媒化されたクリック化学は,タンパク質と望ましくないチオトリアゾールを形成し,研究で偽陽性をもたらします. 新しい方法では,この副産物を最小限に抑え,バイオ・オートゴーナル・ラベリングでより明確な結果が得られます.

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Catalysis02:50

Catalysis

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Preparation of 1° Amines: Azide Synthesis

Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used.
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
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Introduction to Mechanisms of Enzyme Catalysis

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Catalysis01:27

Catalysis

Catalysis influences the rate of chemical reactions by providing an alternative reaction pathway with lower activation energy. A catalyst speeds up a reaction, but it is not consumed during the process. The fundamental principle of catalysis is the ability of a catalyst to alter the reaction mechanism, often introducing a more efficient pathway than the uncatalyzed process.In a catalyzed reaction, the catalyst participates directly in the reaction mechanism. It interacts with reactants to form...
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Heterogeneous Catalysis

Heterogeneous catalysis involves a catalyst in a different phase from the reactants. It is a process where the catalyst and the reactants are in distinct phases, typically solid and gas or liquid.Most heterogeneous catalysts are metals, metal oxides, or acids. The list includes transition metals like iron (Fe), cobalt (Co), nickel (Ni), palladium (Pd), platinum (Pt), chromium (Cr), manganese (Mn), tungsten (W), silver (Ag), and copper (Cu). These metals possess partially vacant d orbitals that...