関連する実験動画

Updated: Jul 5, 2025

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
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Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

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転写-複製の相互作用は,細菌のゲノム調節を明らかにする

Andrew W Pountain1, Peien Jiang1,2, Tianyou Yao3

  • 1Institute for Systems Genetics, NYU Grossman School of Medicine, New York, NY, USA.

Nature
|January 24, 2024
PubMed
まとめ

研究者たちは,細菌のゲノムにおける遺伝子調節を理解するために,トランスクリプション-複製相互作用プロファイル (TRIP) という新しい方法を開発した. このアプローチは,遺伝子発現のダイナミクスが複製と規制要因にどのように影響されているかを明らかにします.

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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
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RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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