受容体関連キナーゼは,植物菌共生における脂質供給プログラムを制御する
Sergey Ivanov1, Maria J Harrison1
1Boyce Thompson Institute, Ithaca, NY 14853, USA.
まとめ
Medicago truncatula の2つのサイクリン依存型キナーゼ型 (CKL) タンパク質は,アーバスキュラー菌根 (AM) の共生に不可欠である. 主要なシグナル受容体との相互作用を通じて,脂質供給と真菌の適応を調節する.
科学分野:
- 植物と微生物の相互作用
- 植物分子生物学
- アーブスキュラー菌糸共生
背景:
- 状菌根 (AM) シンビオシスは,細胞内キノコの適応を必要とする重要な植物-キノコの相互関係です.
- リンパ供給とLysM受容体のようなキナーゼとDMI2を含む特定のシグナル伝達経路は,共生の確立に不可欠である.
研究 の 目的:
- Medicago truncatula AMの共生におけるサイクリン依存キナーゼのような (CKL) タンパク質の役割を調査する.
- CKLタンパク質とDMI2およびLysM受容体キナーズの共生調節における相互作用を解明する.
主な方法:
- Medicago truncatulaの皮質細胞特有のCKLタンパク質の識別と特徴付け
- DMI2とLysM受容体キナーゼによるCKLタンパク質結合とリン酸化の分析
- AM共生と遺伝子発現におけるCKL1とCKL2の機能の遺伝子解析
主要な成果:
- 2つのCKLタンパク質であるCKL1とCKL2は,DMI2とLysM受容体キナーゼと相互作用し, fosforylatedされていることが確認されました.
- CKL1とCKL2は,Medicago truncatulaのAM共生のために不可欠です.
- これらのCKLタンパク質は,脂質供給プログラムに関与する転写因子の発現を調節する.
結論:
- CKL1とCKL2は,AM菌の共生生物に対する植物反応を媒介する上で重要な役割を果たします.
- CKL-DMI2-LysM受容体キナーゼ経路は,脂質供給とエンドシンビオントの収納を調節するために不可欠です.
- この研究は,AM共生における複雑な分子対話を制御する新しい成分を明らかにしています.
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