RiPPバイオシンセシスの非定式アミノ酸の風景を拡大する
Brooke A Johnson1, Kenzie A Clark1, Leah B Bushin1
1Department of Chemistry, Princeton University, Princeton, New Jersey 08544, United States.
Journal of the American Chemical Society
|February 5, 2024
まとめ
研究者らは,Streptococcus pneumoniaeの細菌ペプチドの天然産物で新しい改変を発見しました. この研究は, RiPP とその酵素の既知の多様性を拡張する,異常なアミノ酸の生物合成を詳細に説明しています.
科学分野:
- 微生物学
- 生物化学
- 自然製品化学
背景:
- DNAシーケンシングとバイオインフォマティクスにおける進歩は,微生物における新しい代謝経路を明らかにしています.
- 以前の研究では,Streptococcus pneumoniaeに特有のGRCサブファミリーを含む,ストリプトコックスのRiPPsに対するクオラムセンシング調節遺伝子クラスターのネットワークを特定しました.
研究 の 目的:
- RiPPsのGRCサブファミリーの生合成経路と酵素的修正を解明する.
- GRCクラスター酵素によって生成される新しい非正規のアミノ酸を特徴づける.
主な方法:
- 生体内および体内生化学測定法
- 遺伝子クラスタの識別のための生物情報共存戦略
主要な成果:
- C端のチオラクトンマクロサイクルを設置するThiF型の酵素の解脱.
- L-アロ-Thrとダイデヒドロヒスティジン形成に責任を持つ2つの過激なS-アデノシルメチオニン (RaS) 酵素の特徴.
- GRC製品は2つの非正規のアミノ酸を生成し,エキゾチックなマクロサイクルを特徴とする他のRaS-RiPPとは異なる.
結論:
- GRCクラスターは,RiPP生物合成における既知の非タンパク質性アミノ酸のレパートリーを拡張する.
- 特定された酵素は,天然製品の合成における生物触媒的応用の可能性を提示しています.
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