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関連する概念動画

Nuclear Export01:42

Nuclear Export

3.6K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.7K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.6K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

3.2K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.2K
Regulated mRNA Transport02:22

Regulated mRNA Transport

6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

5.7K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
5.7K

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関連する実験動画

Updated: Jul 3, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.2K

循環型RNAの核輸出

Linh H Ngo1, Andrew G Bert2, B Kate Dredge2,3,4

  • 1RNA Biology and Cancer Laboratory, Peter MacCallum Cancer Centre and Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.

Nature
|February 14, 2024
PubMed
まとめ

研究者らは,円形RNA (circRNAs) の新たな核輸出経路を発見した. この経路は,Ran-GTPによって調節されるエクスポートイン-2とIGF2BP1を利用し,circRNAの調節に関する新しい洞察を提供します.

さらに関連する動画

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.3K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

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関連する実験動画

Last Updated: Jul 3, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.2K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.3K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.5K

科学分野:

  • 分子生物学
  • 細胞生物学
  • RNA 生物学

背景:

  • 円形RNA (circRNAs) は,前体mRNAのバックスプライシングによって形成される.
  • サークRNAは正常な細胞と癌細胞に作用する.
  • サークRNAは主に細胞質であり,核の輸出を必要とします.

研究 の 目的:

  • 円形RNAの核輸出の特定の経路を特定する.
  • サークRNA核輸出を制御する分子メカニズムを解明する.

主な方法:

  • ラン-GTP,エクスポートイン-2,およびIGF2BP1のcircRNAエクスポートにおける役割を調査した.
  • 核Ran-GTPのグラデーションを操作した.
  • エクスポートイン-2のノックアウト/消耗が実行された.
  • 核のcircRNA結合タンパク質とその相互作用を分析した.

主要な成果:

  • サークRNA核の輸出には,Ran-GTP,エクスポートイン-2,およびIGF2BP1を必要とする新しい経路が特定されました.
  • 核Ran-GTPグラデントの調節は,circRNAの輸出率に影響した.
  • エクスポートリン2の減少は,特にcircRNA核のエクスポートを抑制した.
  • Ran- GTPは,IGF2BP1とcircRNAsとの相互作用を強化する.

結論:

  • エクスポートイン-2とIGF2BP1を含むRan-GTP依存経路は,circRNA核のエクスポートを容易にする.
  • このメカニズムはmRNAの輸出とは異なり,タンパク質の輸出に類似しています.
  • IGF2BP1のようなアダプタータンパク質は,circRNAの輸出機構の採用に不可欠です.