Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Antibiotic Selection00:57

Antibiotic Selection

53.5K
Overview
53.5K
tRNA Activation02:26

tRNA Activation

19.2K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
19.2K
Protein Modifications in the RER01:26

Protein Modifications in the RER

5.2K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.2K
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

3.4K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.4K
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

5.7K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.7K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Molecular basis for depsipeptide HDAC inhibitor combinatorial biosynthesis.

Nature communications·2026
Same author

Case Series on the Efficacy of Daratumumab in Treating Patients with Anti-Interferon-Gamma Autoantibodies.

Clinical and experimental immunology·2026
Same author

Exercise Alleviates Osteoporosis and Hyperglycemia in Type 1 Diabetes Mellitus Mice via Piezo1-Mediated Mechanotransduction.

Biology·2026
Same author

Low-intensity Pulsed Ultrasound Ameliorates Obesity-induced Hepatic Lipid Metabolic Disorders.

Ultrasound in medicine & biology·2026
Same author

Synthesis of <i>N</i>-Substituted Hydroxamic Acids through a Pentafluoropyridine-Mediated Procedure.

The Journal of organic chemistry·2026
Same author

PARAS: High-Accuracy Machine Learning of Substrate Specificities in Nonribosomal Peptide Synthetases.

JACS Au·2026

関連する実験動画

Updated: Jul 2, 2025

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
10:41

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Published on: January 13, 2013

18.5K

微分エノイル還元酵素による抗生物質の骨格多様化とトランス-アシルトランスファーゼポリケチド合成酵素におけるモジュールイテレーション

Xinyun Jian1,2,3,4, Fang Pang1, Christian Hobson1

  • 1Department of Chemistry, University of Warwick, Coventry CV4 7AL, U.K.

Journal of the American Chemical Society
|February 23, 2024
PubMed
まとめ
この要約は機械生成です。

グラディオリンとエタンギエン抗生物質の生物合成を調査することで,ポリケチド合成酵素 (PKS) の微妙な違いが構造的多様性を生み出すことが明らかになった. この研究は 微生物の自然産物進化と 工学的な可能性に光を当てています

さらに関連する動画

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.2K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

9.8K

関連する実験動画

Last Updated: Jul 2, 2025

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
10:41

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Published on: January 13, 2013

18.5K
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes T&#252;6028
09:08

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

Published on: January 13, 2017

17.2K
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

9.8K

科学分野:

  • 生物化学と分子生物学
  • 自然製品 化学
  • 微生物学

背景:

  • 複雑な分子構造と選択的生物活性により 微生物の天然製品は 医学や農業に不可欠です
  • これらの化合物の骨格の多様性を生み出す 進化的メカニズムを理解することは 自然製品の研究にとって極めて重要です
  • 生物合成経路の比較分析は,関連する代謝物の構造的変化の起源についての洞察を提供します.

研究 の 目的:

  • 構造的に関連した2つのポリケチド抗生物質であるグラディオリンとエタンジエンの比較生物合成調査を行う.
  • ほぼ同一のポリケチド合成体 (PKS) にもかかわらず,グラディオリンとエタンギエンのC21側鎖の有意な差異に起因するメカニズムを解明する.
  • これらの発見が新しいポリケチド構造の生物合成工学に及ぼす影響を調査する.

主な方法:

  • グラディオリン (Burkholderia gladioli) とエタンジエン (Sorangium cellulosum) の生合成経路の比較分析
  • 簡素化された基質類型を用いた重要な生合成変換の in vitro 再構成.
  • 特定のPKSドメインとスタンドアロン酵素の役割を分析するための遺伝子削除および補足実験.

主要な成果:

  • グラディオリンとエタンジエンを組み立てるポリケチド合成酵素 (PKSs) はほぼ同一であるが,それらのC21側鎖は長さと飽和度において有意な違いを示している.
  • 飽和したグラディオリン側鎖は,モジュール1のシス作用エノイル還元酵素 (ER) ドメインと,PKSのモジュール5へのスタンドアロンERのトランス募集に起因する.
  • グラディオリンPKSのモジュール5は,スタンドアロンERの不在で内在的な反復性を示し, etnangienで観察されたより長いサイドチェーンを説明します.

結論:

  • この研究では,酵素ドメインの微妙な変異と,高度に保存されたPKS内でそれらの採用が,ポリケチドの天然製品における重要な構造的多様性を生み出すことを明らかにした.
  • これらの発見は,グラディオリンとエタンギエン生物合成におけるサイドチェーンの多様化に関するメカニズム的理解を提供します.
  • 得られた洞察は,潜在的な治療用途を持つ新しいポリケチド骨格を作成することを目的とした生物合成技術戦略の進歩に不可欠です.