関連する実験動画
Updated: Jul 2, 2025

07:59
Folding and Characterization of a Bio-responsive Robot from DNA Origami
Published on: December 3, 2015
14.6K
DNA オリガミによる 繰り返し配列の遺伝子定位
Jinxin Xiong1, Zhimei He1, Lianhui Wang1
1State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
Journal of the American Chemical Society
|February 23, 2024
まとめ
研究者は原子力顕微鏡と DNA オリガミを使って 繰り返しのDNA配列を正確に特定する 新しい方法を開発しました この費用対効果の高いテクニックは,既存のシーケンシング技術を補完して,高解像度で遺伝子ローカライゼーションを提供します.
科学分野:
- ゲノミクス
- 分子生物学
- ナノテクノロジー
背景:
- 繰り返される配列はヒトDNAの50%以上を占め 遺伝子の調節に不可欠です
- シーケンシングや光画像のような 現在の検出方法は高価で 時間のかかるもので 繰り返し発生するシーケンスに 精度がないのです
研究 の 目的:
- 繰り返しのシーケンス固有の遺伝子局在化のための正確で費用対効果の高い戦略を開発する.
- DNAの位置と順序を視覚化するための既存の技術の限界を克服する.
主な方法:
- 組み合わせた原子力顕微鏡 (AFM) とDNAオリガミ技術.
- 視覚化のための DNA オリガミタグに繰り返し配列をリンクする"トリブロック"DNA構造を設計した.
主要な成果:
- 高解像度 (約1KHz) を達成した. 6.5 nmまたは19 nt) の繰り返しのシーケンスを視覚化する.
- 遺伝子テンプレート上の異なるDNAの繰り返し配列の同時検出を可能にします.
- 高い効率,精度,そして低操作の複雑さを持つ戦略を示した.
結論:
- 開発された戦略は,繰り返しの配列の正確な遺伝子局所化を提供します.
- この方法は従来のシーケンシング技術に 強力な補足として機能します
- 遺伝子分析と病気診断の費用対効果の高い代替手段です
関連する概念動画
DNA-only Transposons
14.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.5K
Reporter Genes
11.3K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.3K
Conservative Site-specific Recombination and Phase Variation
6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.0K
Gene Duplication and Divergence
6.1K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.1K

