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関連する概念動画

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
The DNA Replication Fork01:02

The DNA Replication Fork

36.0K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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Chromosome Replication02:31

Chromosome Replication

8.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.7K
Replication in Eukaryotes01:29

Replication in Eukaryotes

13.8K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.8K
Homologous Recombination02:31

Homologous Recombination

50.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.5K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
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Updated: Jul 1, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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複製フォークで捕獲された親ヒストンの転送

Ningning Li1, Yuan Gao2, Yujie Zhang3

  • 1State Key Laboratory of Membrane Biology, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing, China.

Nature
|March 6, 2024
PubMed
まとめ
この要約は機械生成です。

研究者たちはFACT複合体と 排出されたヒストンが イーストの複素体と 相互作用する様子を視覚化しました これは,DNA複製中に表遺伝子遺伝を維持するために重要な複製結合ヒストンのリサイクルに関する構造的洞察を示しています.

さらに関連する動画

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

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関連する実験動画

Last Updated: Jul 1, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

351
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

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科学分野:

  • 分子生物学
  • エピジェネティクス
  • 構造生物学

背景:

  • ユカリオットDNAは核細胞を通してクロマチンに圧縮される.
  • ゲノム複製には エピゲノムの調整された伝播が必要です
  • 複製中のヒストンのダイナミクスを理解することは,表遺伝子遺伝の鍵です.

研究 の 目的:

  • DNA複製中のFACT複合体によるヒストンの管理の構造的メカニズムを決定する.
  • 退去したヒストンが 複素体によってどのように処理され 再生されるかを明らかにする.

主な方法:

  • 酵母複素体の冷凍電子顕微鏡 (冷凍EM)
  • 脱出ヒストンとのFACT複合体 (Spt16,Pob3) 相互作用の構造分析
  • Mcm2 ヒストン結合ドメインの相互作用の可視化.

主要な成果:

  • FACT複合体は,リプリソームのフロントで排出されたヒストンを捕捉します.
  • Mcm2はH3-H4テトラマーに結合し,H2A-H2Bの空いた部位を占める.
  • ヒストンは,新たに合成されたDNAへの転送のために再配置され,ヒストンのリサイクルが容易になります.

結論:

  • 複製結合ヒストンのリサイクルに関する重要な構造的洞察を提供します.
  • DNA複製中に表遺伝子情報を保持するメカニズムを示しています.
  • 複製中のヒストンの管理におけるFACTとMcm2の役割を強調する.