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関連する概念動画

The DNA Replication Fork01:02

The DNA Replication Fork

35.9K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.9K
Replication in Prokaryotes01:32

Replication in Prokaryotes

24.9K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
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Replication in Eukaryotes01:29

Replication in Eukaryotes

13.8K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
The Replisome03:01

The Replisome

33.5K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.5K
DNA Replication02:40

DNA Replication

49.5K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

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フォークカップリングはDNA複製の延長と終了を指示します.

Yang Liu1,2, Zhengrong Zhangding1, Xuhao Liu1

  • 1The MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Genome Editing Research Center, Peking University; Beijing 100871, China.

Science (New York, N.Y.)
|March 14, 2024
PubMed
まとめ
この要約は機械生成です。

研究者達は複製のフォークを繋ぐ"噴水のような"DNA構造を発見し,DNA複製がどのように協調されているかを明らかにした. ゲノムの安定性にとって重要なこのプロセスは がんでは破壊され ゲノム削除につながります

さらに関連する動画

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

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関連する実験動画

Last Updated: Jul 1, 2025

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

344
Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

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科学分野:

  • 分子生物学
  • ゲノミクス
  • 細胞生物学

背景:

  • ユカリオットのゲノム複製には DNA複製を多種多様な原点から 早期に開始する必要がある.
  • 複製フォークは起源から双方向に進行し,収束フォークに遭遇すると終了します.
  • 複製フォークの空間的調整を理解することは ゲノムの完全性にとって不可欠です

研究 の 目的:

  • DNA複製のフォークの調整と空間的組織を調査する.
  • 複製中の新生DNAを含むクロマチンの相互作用を捕捉し分析する.

主な方法:

  • 新しい複製関連 in situ HiC 方法の開発
  • 人間とマウスのゲノムにおける新生DNAを含むクロマチンの接触の分析.

主要な成果:

  • クロマチンのコンタクト構造が2000個以上見つかりました DNA複製のフォークが結合していることを示しています
  • 複製フォークの相互作用が姉妹フォークと異なる起源のフォークの間に発生し,終了を容易にする.
  • 終結関連クロマチンの噴水は複製ストレスに敏感であり,がんにおけるゲノム欠損に関連している.

結論:

  • この研究は,クロマチンの文脈内のDNA複製フォークの新しい空間的組織を明らかにしています.
  • 複製フォークカップリングは,事前に決定された複製終了とゲノム安定性において重要な役割を果たします.
  • 複製フォークの調整と関連する構造の障害は,がんのゲノム不安定に寄与する.