Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

E1 Reaction: Kinetics and Mechanism02:46

E1 Reaction: Kinetics and Mechanism

14.6K
Here, in contrast to the E2 reaction mechanism, we delve into the aspects of the E1 reaction mechanism, which has two steps: rate-limiting loss of the leaving group and abstraction of the beta hydrogen by a weak base. Typically, the experimental proof for the E1 mechanism is via kinetic studies or isotope studies. While the former demonstrates the first-order kinetics—the dependence of the reaction solely on substrate concentration—the latter proves the abstraction of hydrogen only...
14.6K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K
Anionic Chain-Growth Polymerization: Mechanism01:04

Anionic Chain-Growth Polymerization: Mechanism

1.7K
The mechanism for anionic chain-growth polymerization involves initiation, propagation, and termination steps. In the initiation step, a nucleophilic anion, such as butyl lithium, initiates the polymerization process by attacking the π bond of the vinylic monomer. As a result, a carbanion, stabilized by the electron‐withdrawing group, is generated. The resulting carbanion acts as a Michael donor in the propagation step and attacks the second vinylic monomer, which acts as a Michael...
1.7K
Toxic Reactions: Overview01:26

Toxic Reactions: Overview

4.3K
When toxic substances penetrate the human body, they disseminate to various tissues, undergoing metabolic changes. This process yields reactive metabolites that may covalently bind with specific target molecules, resulting in toxicity.
Toxicity falls into two primary categories: local and systemic.
Local toxicity appears at the exposure site, such as protein denaturation caused by caustic substances.
In contrast, systemic toxicity requires the toxic agent's absorption and distribution,...
4.3K
Allergic Drug Reactions01:27

Allergic Drug Reactions

1.6K
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
1.6K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Virtual Reality Education for Hospitalized Pediatric Patients Improves Intrinsic Motivation: A Prospective, Randomized Crossover Study.

Clinical pediatrics·2026
Same author

Asymmetric Safety Corridors for Free-Hand S2-Alar-Iliac Screw Placement: Quantifying Direction-Specific Tolerance Around Patient-Specific Optimal Trajectories.

Journal of clinical medicine·2026
Same author

Hybrid Dissolving Microneedles Incorporating Hyaluronic Acid Microdepots for Pain-free and Long-acting Corticosteroid Therapy.

Biomaterials research·2026
Same author

Author Correction: High-throughput discovery of fluoroprobes that recognize amyloid fibril polymorphs.

Nature chemistry·2026
Same author

Brain-Immune Crosstalk in Mental Illness: The Path to Immunopsychiatry.

Biological psychiatry·2026
Same author

Impact of CYP and ABCB1 Polymorphisms on Bortezomib-Induced Adverse Events in Multiple Myeloma.

Biomedicines·2026
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: May 6, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

15.9K

I型インターフェロン反応性マイクログリア 皮質の形状 発達と行動

Caroline C Escoubas1, Leah C Dorman2, Phi T Nguyen3

  • 1Departments of Psychiatry and Behavioral Sciences/Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA 94158, USA.

Cell
|March 15, 2024
PubMed
まとめ
この要約は機械生成です。

I型インターフェロン (IFN-I) は,脳の発達中にマイクログリアを活性化し,ニューロンを吸収します. IFN-I信号の喪失は,このプロセスを阻害し,過剰なニューロンと感覚過敏を引き起こします.

キーワード:
皮質の発達マイクログリア神経免疫性ファゴサイトーシス体感覚皮質タクティルの過敏症I型インターフェロン

さらに関連する動画

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
07:54

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

9.8K
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
10:40

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

32.0K

関連する実験動画

Last Updated: May 6, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

15.9K
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
07:54

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

9.8K
Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain
10:40

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Published on: October 27, 2019

32.0K

科学分野:

  • 神経科学
  • 免疫学
  • 発達生物学

背景:

  • 脳の免疫細胞であるマイクログリアは 神経発達において重要な役割を果たし 神経発達障害と関連しています
  • 異なるマイクログリア状態は存在しますが,その機能的な役割はほとんど不明です.

研究 の 目的:

  • 神経回路の発達に関与する特定のマイクログリア状態を特定し,特徴づけること.
  • I型インターフェロン (IFN-I) 信号伝達のマイクログリア活動と脳の発達における機能的意義を解明する.

主な方法:

  • 発達中の体感皮質におけるマイクログリアの転写プロファイリング.
  • in vivoモデル (マウスとゼブラフィッシュ) を用いてニューロンのマイクログリアル吸収を調査する.
  • 遺伝子操作 (IFN-I受容体の機能の喪失と獲得) と実験的刺激 (ひげの剥奪) を利用する.

主要な成果:

  • I型インターフェロン (IFN-I) に反応するマイクログリアル群が特定され,発達中の皮質のニューロンを積極的に吸収している.
  • IFN- I受容体の欠乏はマイクログリアルファゴリスオーム機能障害とDNA損傷ニューロンの蓄積につながった.
  • IFN-Iの機能増強はニューロンの吸収を高め,DNA損傷ニューロンを減少させた.
  • IFN- I欠乏は皮質刺激ニューロンと触覚過敏を過剰に導いた.

結論:

  • 神経を包み込むマイクログリアは,IFN-Iシグナル伝達によって制御され,脳発達の重要な窓の間に重要な役割を果たします.
  • 常識的な抗ウイルスIFN-I経路は,発達中の脳内でホメオスタティックな機能を発揮し,ニューロン集団と感覚処理に影響を与えます.