関連する実験動画
Updated: Jun 29, 2025

11:14
Primer-Free Aptamer Selection Using A Random DNA Library
Published on: July 26, 2010
24.8K
合成ポリマーの混合シーケンスライブラリにおける選択的二重形成
Mohit Dhiman1, Ronan Cons1, Oliver N Evans1
1Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.
Journal of the American Chemical Society
|March 26, 2024
まとめ
認識コードメラミンオリゴーマー (REMO) の自動合成により,シーケンス固有の二重構造が形成されます. この方法は,複雑な混合物でも,合成ポリマーの高信頼性自己組み立てを可能にします.
科学分野:
- ポリマー化学
- 超分子化学
- 有機合成
背景:
- 認識コードメラミンオリゴーマー (REMO) は,定義された骨格と特定の認識単位を持つ合成ポリマーである.
- 以前の方法は,複雑なポリマーライブラリのための自動化された,シーケンス固有の合成能力が欠けていました.
研究 の 目的:
- 任意の指定されたシーケンスでREMOのための自動固相合成 (SPS) 方法を開発する.
- REMOのセルフアセンブリとデュプレックス形成を,特に混合シーケンスライブラリで調査する.
主な方法:
- ダイクロトリアジン単体構造ブロックを用いたREMOの自動固体合成
- 補完性ホモオリゴマーの合成とNMRデナチュレーションによるダプレックス形成の確認.
- 末端グループ機能化後の銅触媒アルキンアジドサイクル添加 (CuAAC) を使用した二重複素の共性トラッピング.
- 混合シーケンスライブラリ合成と自己組み立て特性のスクリーニングのためのSPSの適応.
主要な成果:
- REMO合成のための堅固な自動化されたSPS方法が確立されました.
- コンプリメンタリーなホモオリゴーマーが非極性溶媒で安定した二重体を形成する.
- 混合シーケンスライブラリのスクリーニングは,マイクロモラー濃度で高精度,シーケンス選択の二重形成を示した.
- CuAAC反応は 機能性スクリーニングを可能にする 自己組み立てのデュプレックスを効果的に閉じ込めました
結論:
- 自動合成アプローチは,多様なREMOライブラリへの効率的なアクセスを提供します.
- 配列補完のREMOオリゴーマーには,統計的混合物の中にさえも,正確な自己組み立てがある.
- この方法論は,合成ポリマーの配列依存特性の研究を容易にする.
さらに関連する動画
関連する概念動画
Maxam-Gilbert Sequencing
11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Challenges of the Maxam-Gilbert Method
The...
11.2K
Next-generation Sequencing
88.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.7K
Lagging Strand Synthesis
51.4K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
51.4K
Sanger Sequencing
754.2K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.2K
Translesion DNA Polymerases
10.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
10.0K
Single-Strand DNA Binding Proteins
14.1K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.1K

