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Updated: Jun 29, 2025

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

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ディメチルリジンの翻訳後の改変の選択的標識のための酸化による三次アミン結合

Benjamin Emenike1, Patrick Czabala1, Jonathan Farhi2

  • 1Department of Chemistry, Emory University, Atlanta, Georgia 30322, United States.

Journal of the American Chemical Society
|April 8, 2024
PubMed
まとめ

研究者は,リジン二甲基化 (Kme2) 後の改変を選択的に標識するための新しい化学方法である酸化による三次アミン結合 (TACO) を開発した. この画期的な発見により 複雑な生物学的サンプルで Kme2 の部位を 全球的に特定できます

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond.
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
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