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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.1K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.1K
Heterochromatin02:38

Heterochromatin

12.5K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
12.5K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.3K
Euchromatin01:01

Euchromatin

6.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
6.9K

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関連する実験動画

Updated: Jun 26, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

18.0K

ゲノタイプを単細胞のクロマチンアクセシビリティプロファイルにマッピングする

Franco Izzo1,2,3,4, Robert M Myers5,6,7,8, Saravanan Ganesan5,6,7

  • 1New York Genome Center, New York, NY, USA. franco.izzo@mssm.edu.

Nature
|May 8, 2024
PubMed
まとめ

ソマティック変異はクロマチンのアクセシビリティを変化させ,細胞の分化に影響します. 新しい方法であるGoT-ChAは,JAK2V617F変異が血幹細胞の表遺伝子を改造し,炎症を引き起こし,細胞の運命を変化させることを明らかにしています.

さらに関連する動画

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

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関連する実験動画

Last Updated: Jun 26, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

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科学分野:

  • エピジェネティクスとゲノミクス
  • ヘマトポエシス
  • 癌 生物学

背景:

  • クロマチンのアクセシビリティの変化は細胞の分化に不可欠です.
  • 体の変異はこれらのパターンを破壊し,異常な細胞の成長につながります.
  • ヒトのサンプルにおける突然変異の影響の研究は,混合細胞タイプのため困難である.

研究 の 目的:

  • ヒトのクローン産生における体質変異が表遺伝的景観にどのように影響するかを分析する方法を開発する.
  • 骨髄増殖性腫瘍におけるクロマチンのアクセシビリティに対するJAK2V617F変異の影響を調査する.

主な方法:

  • 単細胞クロマチンのアクセシビリティ (GoT-ChA) を有する標的ロキのゲノタイプ化を開発し,単細胞解像度でのクロマチンのアクセシビリティとゲノタイプを関連付けました.
  • JAK2V617F変異性骨髄増殖性腫瘍患者のCD34+細胞にGoT- ChAを適用した.
  • プラットフォーム (DOGMA-seq) を拡張し,RNA発現と細胞表面タンパク質分析を含めた.

主要な成果:

  • JAK2V617F変異性血液生成前体における細胞内および細胞状態特有の表遺伝子シフトを特定した.
  • 造血幹細胞の炎症誘発性シグネチャーと,メガカリオシトの原始細胞の炎症誘発性シグネチャーを観察した.
  • JAK2V617Fの変異は 細胞内にある 細胞型特異の表皮遺伝的再配線を 引き起こしていることが示されました

結論:

  • JAK2V617Fのような体内変異は,細胞特異的な方法で重要な表遺伝子変異を引き起こす.
  • これらのエピジェネティックの変化は炎症と細胞の分化経路に影響します.
  • GoT-ChAプラットフォームは,様々な文脈で体内の変異と表遺伝学を研究するための強力なツールを提供します.