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関連する概念動画

Thermosensation01:43

Thermosensation

30.4K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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Increased Body Temperature01:25

Increased Body Temperature

664
A body temperature above  38°C  (100.4 °F) is known as fever or pyrexia, and a person with fever is termed 'febrile.' Typically, the hypothalamus, a part of the brain that acts as the body's thermostat, regulates body temperature through a thermoregulatory setpoint. It receives signals from cold and warm thermal receptors throughout the body and adjusts the body's temperature accordingly. Fever occurs when this hypothalamic setpoint is altered, usually in...
664
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

2.2K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.2K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

6.4K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
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G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

4.6K
GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
4.6K
Body Temperature01:25

Body Temperature

941
The body's temperature, measured in degrees, is determined by the balance between heat production and dissipation to the surrounding environment. For instance, if exercising vigorously, the body will produce more heat, causing sweat and dissipating that heat. Despite extreme environmental conditions and physical exertion, the human temperature-control system maintains a constant core body temperature (the temperature of deep tissues, which are the tissues located beneath the skin and other...
941

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関連する実験動画

Updated: Jun 26, 2025

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

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生理学的温度駆動 TRPM4 リガンド認識とゲーティング

Jinhong Hu1, Sung Jin Park1, Tyler Walter1,2

  • 1Van Andel Institute, Grand Rapids, MI, USA.

Nature
|May 15, 2024
PubMed
まとめ

生理学的温度でTRPM4イオンチャネルを研究すると,

さらに関連する動画

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
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Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

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関連する実験動画

Last Updated: Jun 26, 2025

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

14.8K
Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

10.1K
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

8.0K

科学分野:

  • バイオ物理学
  • 構造生物学
  • イオンチャンネル生理学

背景:

  • マクロ分子機能,特にタンパク質の活動は,温度によって大きく影響されます.
  • 生物物理学的研究はしばしば生理学的温度を無視し,潜在的に不正確な生物学的洞察を生み出します.
  • TRPM4のような温度に敏感なイオンチャネルは 生理学的プロセスにおいて重要な役割を果たします

研究 の 目的:

  • 温度に依存するTRPM4イオンチャネルの構造的および機能的特性を調査する.
  • 生理学的温度でTRPM4がどのように機能し,リガンドと相互作用するかを理解する.
  • 生理学的に重要な熱条件下でのTRPM4のゲートメカニズムを解明する.

主な方法:

  • 生理学的温度でのTRPM4の単粒子の冷凍電子顕微鏡 (冷凍EM)
  • 異なる形状を特定するための構造分析
  • リガンド結合とチャネルゲーティングを評価する機能的測定法.

主要な成果:

  • 低温構造と異なる生理学的温度でTRPM4の新しい"暖かい"構造が特定されました.
  • 細胞内領域の温度に依存するカルシウム結合部位は,TRPM4の機能に不可欠であることが判明した.
  • デカバナダートとATPのリガンド結合部位は温度に依存し,機能的関連性があることが示された.
  • TRPM4のゲートメカニズムは解明され,これまで観察されなかった生理的な温度でチャネルが開くことが明らかになった.

結論:

  • TRPM4は生理的な温度で独特の構造的および機能的特性を表しています.
  • 物理的な温度でマクロ分子,特にイオンチャネルを研究することは,正確なメカニズムと薬理学的な理解に不可欠です.
  • この発見は,熱感性TRPMチャネルの温度知覚を理解するための分子枠組みを提供します.