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Updated: Jun 23, 2025

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

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モノマー/ディマー混合物のスピンラベル間のモノマーおよびディマー特異的距離分布を解析T1-編集されたDEER EPRスペクトロシー

Thomas Schmidt1, Nina Kubatova1, G Marius Clore1

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520, United States.

Journal of the American Chemical Society
|June 18, 2024
PubMed
まとめ

この研究では,ダブル電子共振 (DEER) と電子パラマグネット共振 (EPR) の縦断リラクゼーション (T1) 実験を組み合わせた新しい方法が導入されています. このテクニックは,複合混合物における単体および二元タンパク質種をうまく区別し,距離を割り当てる.

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