FilamentIDは,ゲメトゲネシス中の代謝酵素ポリマーの組成と機能を明らかにする

Jannik Hugener¹, Jingwei Xu², Rahel Wettstein³

¹Institute of Molecular Biology and Biophysics, ETH Zürich, 8093 Zürich, Switzerland; Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland; Max Perutz Labs, University of Vienna, 1030 Vienna, Austria.

Cell

|June 21, 2024

まとめ

細胞は休眠状態でユニークな繊維を形成しますこの構造はFilamentIDを使って酵母胞子が回復し飢餓後の細胞循環に戻るのを助けます

科学分野:

細胞生物学
構造生物学
酵母遺伝学

背景:

細胞の休眠状態は生殖に不可欠ですが回復メカニズムは不明です
芽生えた酵母細胞は,ゲメット形成と胞子発育の間に発育を停止します.

研究の目的:

芽生えた酵母菌の細胞回復の構造的基礎を調査する.
微分化と胞子発達の過程で観察された有糸組の分子構成と機能を特定する.

主な方法:

高解像度イメージングのための冷凍電子トモグラフィー (cryoET) と冷凍電子顕微鏡 (cryoEM).
マルチスケール画像と分析を組み合わせた新しい"フィラメント識別" (FilamentID) ワークフロー.
糸の形成と細胞の回復を研究する遺伝的混乱

主要な成果:

酵母細胞の核,細胞質,ミトコンドリアで糸状の集合体を発見した.
アルデヒド脱水素酵素Ald4 (ALDH2) で構成されているミトコンドリアの繊維を特定した.
アセチル-コア合成酵素Acs1 (ACSS2) として核プラズマ/細胞プラズマフィラメントを特定し,ポリメリゼーションメカニズムを明らかにした.
Acs1のポリメリゼーションは,老いた胞子の回復と,飢えた細胞の細胞サイクル再侵入を助長することを示した.

結論:

FilamentIDは未知の細胞フィラメントを特徴付ける強力なツールです
Acs1のポリメリゼーションは,細胞の休眠状態と飢餓状態からの回復に重要な役割を果たします.
これらのフィラメント構造を理解することで基本的な細胞生存戦略の洞察が得られます

キーワード:

細胞のストレスインサイト構造統合的構造生物学メタボリズム方法開発マルチスケールイメージングタンパク質繊維静止する飢え

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