関連する実験動画

Updated: Jun 22, 2025

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
12:02

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Published on: April 18, 2014

20.8K

効率的な植物ゲノム工学のためのトランポゼーゼ支援のターゲットサイト統合

Peng Liu1, Kaushik Panda1, Seth A Edwards1,2

  • 1Donald Danforth Plant Science Center, St Louis, MO, USA.

Nature
|June 26, 2024
PubMed
まとめ

科学者たちは,植物のゲノムにDNAを正確に挿入するために,移植可能な要素 (TE) を使用して新しいゲノムエンジニアリングツールを開発しました. この画期的な発見は 過去の限界を克服し 標的型DNAの挿入によって 効率的な作物の改良を可能にしました

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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

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Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants08:48

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

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関連する概念動画

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Transgenic Organisms00:53

Transgenic Organisms

Overview
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Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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