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Updated: Jun 22, 2025

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

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ホスト-ゲストの相互作用による細胞フリーシステムにおけるゲスト修正アデノシンによる遺伝子発現の可逆制御

Hidenori Okamura1,2, Takeyuki Yao1,2, Fumi Nagatsugi1,2

  • 1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.

Journal of the American Chemical Society
|June 28, 2024
PubMed
まとめ

研究者らは,宿主-ゲスト化学を用いて,細胞のないシステムにおける遺伝子発現を制御する新しい方法を開発した. この突破は,高度なバイオテクノロジーの応用のために 転写とタンパク質の生産を 精密かつ可逆的に制御することを可能にします

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Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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