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関連する概念動画

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

825
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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¹H NMR: Long-Range Coupling01:27

¹H NMR: Long-Range Coupling

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The coupling interactions of nuclei across four or more bonds are usually weak, with J values less than 1 Hz. While these are usually not observed in spectra, the presence of multiple bonds along the coupling pathway can result in observable long-range coupling.
In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene...
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Cycloaddition Reactions: MO Requirements for Thermal Activation01:16

Cycloaddition Reactions: MO Requirements for Thermal Activation

3.5K
Thermal cycloadditions are reactions where the source of activation energy needed to initiate the reaction is provided in the form of heat. A typical example of a thermally-allowed cycloaddition is the Diels–Alder reaction, which is a [4 + 2] cycloaddition. In contrast, a [2 + 2] cycloaddition is thermally forbidden.
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Pericyclic Reactions: Introduction01:17

Pericyclic Reactions: Introduction

8.3K
Pericyclic reactions are organic reactions that occur via a concerted mechanism without generating any intermediates. The reactions proceed through the movement of electrons in a closed loop to form a cyclic transition state, where rearrangement of the σ and π bonds yields specific products.
Pericyclic reactions can be classified into three categories: electrocyclic reactions, cycloaddition reactions, and sigmatropic rearrangements. Electrocyclic reactions and sigmatropic...
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Valence Bond Theory and Hybridized Orbitals02:38

Valence Bond Theory and Hybridized Orbitals

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According to valence bond theory, a covalent bond results when: (1) an orbital on one atom overlaps an orbital on a second atom, and (2) the single electrons in each orbital combine to form an electron pair. The strength of a covalent bond depends on the extent of overlap of the orbitals involved. Maximum overlap is possible when the orbitals overlap on a direct line between the two nuclei.
A σ bond (single bond in a Lewis structure) is a covalent bond in which the electron density is...
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Updated: Jun 20, 2025

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
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形を補うシドオサイクルを用いて様々な小さな分子を結合し,感知する

Linna An1,2, Meerit Said1,2, Long Tran2,3,4

  • 1Department of Biochemistry, University of Washington, Seattle, WA, USA.

Science (New York, N.Y.)
|July 18, 2024
PubMed
まとめ
この要約は機械生成です。

感知用の小さな分子結合タンパク質を 設計するための ディープラーニングの方法を開発しました このアプローチにより,様々な分子に高親密性の結合物質が作られ,新しいセンサー技術が可能になります.

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Last Updated: Jun 20, 2025

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

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Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
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科学分野:

  • タンパク質工学
  • バイオテクノロジー
  • 計算生物学

背景:

  • 特定の小分子結合能力を持つ タンパク質を設計することは 難しいことです
  • 既存の方法は様々な分子標的に対して 汎用性が欠けていることが多い.

研究 の 目的:

  • 高親和性小分子結合タンパク質を設計するための新しい計算手法を開発する.
  • センサーや化学的に誘発された二分化などの 下流アプリケーションに適したタンパク質を作成します

主な方法:

  • 調整可能な結合ポケットの形を持つ タンパク質の擬似サイクルを生成するために ディープラーニングを利用した.
  • 標的小分子に対する補完的なタンパク質の設計を特定するために計算ドッキングを使用した.
  • 高結合親和度のための最適化された相互作用表面と実験的なスクリーニングを行いました.

主要な成果:

  • メトトレキサートとチロキシンを含む4つの異なる小分子に対する高親和結合剤を成功裏に設計し,検証した.
  • 設計されたタンパク質のモジュール性を化学的に誘導された二元化システムを作成することによって示した.
  • 設計されたタンパク質ドメインを利用した低騒音ナノ孔センサーを設計した.

結論:

  • ディープラーニングベースの偽回路設計アプローチは,高親和性小分子結合体を作成するのに有効です.
  • タンパク質のモジュラーデザインは バイオセンシングと分子制御システムの開発を容易にする.
  • この方法は,広範なバイオテクノロジーの可能性を持つタンパク質工学のための汎用的なプラットフォームを提供します.