Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

8.0K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
8.0K
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

3.9K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
3.9K
Enzymes02:34

Enzymes

81.3K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
81.3K
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation02:17

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation

3.3K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.3K
Catalysis02:50

Catalysis

26.8K
The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
26.8K
Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration02:34

Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration

8.4K
The rate of acid-catalyzed hydration of alkenes depends on the alkene's structure, as the presence of alkyl substituents at the double bond can significantly influence the rate.
8.4K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

A highly stable potentiometric Li<sup>+</sup>-sensing chip based on hydrophobic laser-induced graphene.

Chemical communications (Cambridge, England)·2026
Same author

Toward Reliable Environmental Monitoring with Potentiometric Ion Sensors: Unveiling the Effects of Humic Acids and Developing Antifouling Sensors Based on a Poly(vinylidene fluoride) Coating.

Analytical chemistry·2026
Same author

Versatile polymeric membrane ion-selective electrodes based on cellulose triacetate.

The Analyst·2026
Same author

Polymeric Membrane Potentiometric Ion Sensors with Dual Biocompatible Functionality.

Analytical chemistry·2025
Same author

Dual-redox functionalized porous graphene for highly reproducible and stable solid-contact ion-selective electrodes.

Chemical communications (Cambridge, England)·2025
Same author

Sensor-Aided Rapid Identification of Plastic-Degrading Bacterial Strains.

Analytical chemistry·2025

関連する実験動画

Updated: Jun 18, 2025

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

5.4K

微小滴媒介酵素触媒における選択性の向上

Yinhao Li1,2, Jiawang Ding1,2, Wei Qin1,3,4,2

  • 1CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, Shandong 264003, P. R. China.

Journal of the American Chemical Society
|July 29, 2024
PubMed
まとめ

マイクロドロップレットは 活性部位の電場を活性化することで 酵素の触媒を活性化します このシンプルな方法は酵素の選択性を向上させ,バイオセンシングとバイオシンセシスのためのグリーンな解決策を提供します.

さらに関連する動画

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

5.7K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

9.5K

関連する実験動画

Last Updated: Jun 18, 2025

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

5.4K
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

5.7K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

9.5K

科学分野:

  • 生物化学
  • 化学工学
  • 物理化学

背景:

  • 酵素触媒は生物学的プロセスや産業用途において極めて重要です
  • 酵素の微小環境を調整することは,活性と選択性を高めるための一般的な戦略です.
  • しかし,アクティブサイトの電場を調節することは,依然として大きな課題です.

研究 の 目的:

  • 酵素の活性部位の電場を高めるための単純な原子炉としてマイクロドロップルの使用を調査する.
  • マイクロドロップレット媒介の触媒を用いた酵素選択性の改善を証明する.
  • 微小粒子が酵素の静電と触媒経路に影響を与えるメカニズムを解明する.

主な方法:

  • モデル酵素としてホースラディッシュペロキシデスを利用した.
  • ミクロドロップレットが反応媒介として使われています
  • 量子力学/分子動力学の計算を行いました.
  • 振動のスタークスペクトロスコーピーを行いました

主要な成果:

  • マイクロドロップレットは酵素の活性部位の電場を高めることが示された.
  • マイクロドロップレット媒介のホースラディッシュペロキシダース触媒で選択性の改善が観察されました.
  • 計算とスペクトロスコピーは,マイクロドロップレットインターフェースの電場が酵素の前組織化と内部電場強度に影響することを明らかにしました.
  • 基質とヘムフリーエネルギーの調節により 触媒経路が変化し,選択的なC-N添加が可能になった.

結論:

  • マイクロドロップレットは 酵素触媒反応を 調節するための シンプルで 緑色で 効果的な方法を提供します
  • このアプローチは活性部位の電場に影響を与えることで 酵素の選択性を高めます
  • この発見は,バイオセンシングとバイオシンセシスの応用に重大な意味を持つ.