Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Replication in Eukaryotes01:29

Replication in Eukaryotes

13.6K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.6K
The DNA Replication Fork01:02

The DNA Replication Fork

35.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.7K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
Replication in Prokaryotes01:32

Replication in Prokaryotes

24.8K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.8K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

4.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.7K
Genome Copying Errors02:46

Genome Copying Errors

4.2K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.2K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Oral ceramide attenuates ultraviolet Binduced epidermal dysregulation and hyperpigmentation by modulating MITF signaling and barrier-related pathways.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Cytoplasmic competition between separate parental pronuclei in zygotes.

Nature·2026
Same author

Molecular basis of chromosome segregation and age-associated errors in oocyte meiosis I.

Biology of reproduction·2026
Same author

Dynamic blebbing and absence of organelle transfer during mouse oocyte formation.

The EMBO journal·2026
Same author

scRepli-RamDA-seq: a multi-omics technology enabling the analysis of gene expression dynamics during S-phase.

Nature communications·2025
Same author

Particulate thiols along a meridional transect in the western North Pacific: Insights from laboratory cultures of Synechococcus sp. and Thalassiosira nordenskioeldii.

The Science of the total environment·2025
Same journal

Six ways to put the public at the heart of science and policy.

Nature·2026
Same journal

The complex truth about trust in science.

Nature·2026
Same journal

Have people stopped trusting science? The data tell a surprising story.

Nature·2026
Same journal

How FAIR data are helping to build trust in science.

Nature·2026
Same journal

Scientists should recognize their own political biases to build public trust.

Nature·2026
Same journal

Harmonizing standards and resources for the medical genome.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: Jun 14, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.4K

DNA複製のタイミングプログラム出現時に胚のゲノム不安定性

Saori Takahashi1, Hirohisa Kyogoku2,3, Takuya Hayakawa4

  • 1Laboratory for Developmental Epigenetics, RIKEN Center for Biosystems Dynamics Research (BDR), Kobe, Japan.

Nature
|August 28, 2024
PubMed
まとめ
この要約は機械生成です。

初期のマウス胚は DNAの複製が調整されていないために ゲノムが一時的に不安定になったことを示している. 複製の遅いフォークとDNAの損傷によって特徴づけられるこの不安定性は,8細胞段階によって解決され,ゲノムの完全性を確保します.

さらに関連する動画

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

13.9K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.8K

関連する実験動画

Last Updated: Jun 14, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.4K
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

13.9K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.8K

科学分野:

  • 発達生物学
  • 遺伝学
  • 分子生物学

背景:

  • 忠実にDNAを複製することは ゲノムの整合性を維持するために不可欠です
  • 複製の欠陥と染色体分離の誤りは,初期の胚形成で観察されています.
  • 初期の哺乳類の胚におけるDNA複製の調節は十分に理解されていません.

研究 の 目的:

  • マウス胚のDNA複製プログラムを 単細胞レベルで研究する
  • 初期の発達期における ゲノム不安定の臨界期を特定する
  • 複製のタイミングとフォークの進行の調整を理解する.

主な方法:

  • マウス胚における単細胞,全ゲノムDNA複製アトラスの構築.
  • 複製タイミングプログラムと複製フォークの速度を分析する.
  • 複製ストレス,DNA損傷,染色体分離エラーの評価

主要な成果:

  • 初期の胚 (1-2 細胞) は,ゆっくりと均一な複製で複製のタイミングプログラムが欠けている.
  • ソマティック型の複製プログラムは 4 細胞段階から始まりますが,緩やかなフォークと複製のストレスが増加します.
  • 破裂型染色体分離の誤差は,4〜8の細胞分裂の間に発生し,後期複製領域に関連しています.
  • 核酸サプリメントはフォークの速度を加速し,ストレスを軽減することによってエラーを救済します.
  • 8細胞段階では 複製の動態が正常化し 染色体の異常が減少します

結論:

  • マウスの正常な発達過程で ゲノム不安定が一時的に起こる.
  • この不安定さは,早期のS段階における複製のタイミングとフォークの調整の欠如に関連しています.
  • 複製プロセスの調整は,胚形成の間にゲノムの安定性を維持するために不可欠です.