関連する実験動画

Updated: Jul 11, 2026

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
06:45

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Published on: May 26, 2011

タンパク質のアンカリングと細胞表面輸送のための膜横断ドメインの構造要件

G A Adams, J K Rose

    Cell
    |July 1, 1985
    PubMed
    まとめ

    膀性口炎ウイルスにおけるトランスメブランドメインの大きさは,その適切な細胞表面輸送のために,グリコプロテイン (G) が不可欠である. このドメインの縮小は,膜の固定と細胞内の正常なタンパク質の輸送に影響します.

    さらに関連する動画

    Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies12:05

    Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

    Published on: March 6, 2013

    Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy10:49

    Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

    Published on: March 5, 2017

    関連する概念動画

    Lipids as Anchors01:32

    Lipids as Anchors

    In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
    The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
    Single-pass Transmembrane Proteins01:25

    Single-pass Transmembrane Proteins

    Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
    Membrane Domains01:18

    Membrane Domains

    The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
    Protein Domains
    The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
    GPI Anchoring of Proteins in the ER Membrane01:29

    GPI Anchoring of Proteins in the ER Membrane

    GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
    GPI-anchor structure
    A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
    Multi-pass Transmembrane Proteins and β-barrels01:09

    Multi-pass Transmembrane Proteins and β-barrels

    In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
    α-Helix containing multi-pass transmembrane proteins
    Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...
    Mechanisms of Membrane Domain Formation00:59

    Mechanisms of Membrane Domain Formation

    Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
    Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...