関連する実験動画
Updated: Jun 13, 2025
05:44
Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
Published on: January 26, 2024
788
人間のTIP60-Cヒストン交換およびアセチルトランスフェラーゼ複合体の構造
Changqing Li1,2,3,4, Ekaterina Smirnova1,2,3,4, Charlotte Schnitzler1,2,3,4
1Université de Strasbourg, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC) UMR 7104 UMR S 1258, Illkirch, France.
Nature
|September 11, 2024
まとめ
人間のTIP60-EP400複合体 (TIP60-C) は,酵母SWR1とNuA4の活動を三葉構造に統合しています. このクロマチンの調節器は,酵母複合体とは異なる新しいアクティベーターベースの徴用メカニズムを使用しています.
科学分野:
- 分子生物学
- クロマチン生物学
- 構造生物学
背景:
- 染色体構造は,転写,複製,修復などの DNA の重要なプロセスを制御します.
- 人間のTIP60- EP400複合体 (TIP60- C) は,ヒストンH2A- H2B交換とヒストンアセチル化という二重の酵素活性を持っています.
- イーストでは,これらの活動は人間のスーパーコンプレックスとは異なり,独立したSWR1とNuA4複合体に分離されます.
研究 の 目的:
- ヒトの内生性TIP60-Cの構造を明らかにする.
- 結合されたSWR1とNuA4の活動がタンパク質構造とクロマチンの採用にどのように影響するかを理解する.
- 人間におけるヒストンの交換と複合組成のメカニズムを調査する.
主な方法:
- TIP60-Cの構造を決定するための冷凍-EMまたはX線結晶学.
- TIP60-Cの活性とヒストンの交換メカニズムをテストする生化学的測定法
- 核細胞結合を視覚化して酵母と比較する計算モデルです.
主要な成果:
- 人間のTIP60-Cは,SWR1型とNuA4型のコンポーネントを備えた3つのロブアーキテクチャを持ち,EP400によってエスカフォールドされています.
- TRRAPのアクティベーター結合モジュールは イーストのモジュールとは異なり 柔軟に結合されています
- ヒストン交換メカニズムとクロマチンの徴集は,固定アクチンモジュールとアクティベータベースのモードを含む酵母と異なる.
結論:
- ヒトのTIP60-Cは酵母と比べて 独特の構造とメカニズムを持つ 超複合体です
- EP400は,異なる機能モジュールを統合する中心的な支架として機能します.
- TIP60-Cは,酵母モデルとは異なるアクティベータ依存のクロマチン標的化戦略を採用しています.
さらに関連する動画
07:26Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
Published on: December 26, 2020
4.0K
09:43Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
Published on: November 30, 2018
21.3K
関連する概念動画
02:32Histone Modification
13.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.2K
01:12The Nucleosome Core Particle
892
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
892
02:25Spreading of Chromatin Modifications
8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.2K
02:54Nucleosome Remodeling
9.0K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.0K
02:30Histone Variants at the Centromere
4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K
01:19The Nucleosome
1.4K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
1.4K