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関連する概念動画

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)01:20

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)

983
Two NMR-active nuclei bonded to a central atom can be involved in geminal or two-bond coupling. Geminal coupling is commonly seen between diastereotopic protons in chiral molecules and unsymmetrical alkenes, among others.
The central atom need not be NMR-active because its electrons are affected by the electron polarization of the spin-active atoms. However, spin information is transmitted less effectively than in one-bond coupling, and 2J values are usually weaker than 1J values. The energy of...
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G Protein-coupled Receptors01:15

G Protein-coupled Receptors

11.5K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
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Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

6.8K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
6.8K
DNA Base Pairing02:27

DNA Base Pairing

27.2K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
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G-protein Coupled Receptors01:21

G-protein Coupled Receptors

117.1K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
117.1K
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

1.9K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
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関連する実験動画

Updated: Jun 13, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
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Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

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G-Triad•G接続を用いたG-クアドルプレックスの相互接続:Gワイヤアセンブリへの影響

Abhishek Singh1, Fernaldo Richtia Winnerdy1, Constanza Avendaño Avila2

  • 1School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.

Journal of the American Chemical Society
|September 14, 2024
PubMed
まとめ

この研究では,新しいG-トライアード-G相互作用で接続された,空いた場所と余分なグアニンを持つG-クアドルプレックス構造が相互に絡み合っていることが明らかになりました. この発見により ナノテクノロジーの自己組み立てGワイヤが可能になりました

さらに関連する動画

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

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関連する実験動画

Last Updated: Jun 13, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
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Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

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科学分野:

  • 生物化学
  • ナノテクノロジー
  • 構造生物学

背景:

  • G四重複体は,細胞プロセスとナノテクノロジーにとって不可欠な非正規の核酸構造です.
  • 最近の研究では,G-テトラド核に欠陥があるものを含め,新しいG-四重複構造を発見しました.

研究 の 目的:

  • 2つの異なる分子内G四重複体の相互接続を証明する
  • 空白部位と結合していないグアニンの間の相互作用メカニズムを探求する.
  • ナノ技術の応用のためにGワイヤに自己組み立て可能な配列を特定する.

主な方法:

  • 空白部位 (4n-1) と無結合グアニン (4n+1) を含む分子内G四重複体を研究する.
  • G-トライアード-Gの接続と5'-3'のスタッキングの相互作用を分析する.
  • カリウム (K+) 溶液における自己組み立てG線形成の特徴

主要な成果:

  • G-トライアード-Gリンクを通して2つの内分子G-四重複の相互接続を証明した.
  • 前例のない5'-3'の積み重ねが 相互接続構造に観測されました
  • K+溶液でG線に自己組み立てられる特定の配列を特定した.

結論:

  • G四重複体の新しい相互接続メカニズムは 核酸構造の理解を広げています
  • 特定されたG-ワイヤ形成配列は,将来のナノテクノロジーの応用に有望である.
  • この研究は 構造生物学的な洞察と 潜在的技術的進歩を結びつけています