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Updated: Jun 13, 2025

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生細胞における核フコシライゼーションの化学酵素によるラベル付け,検出およびプロファイリング

Qiang Zhu1, Jean-Luc Chaubard2, Didi Geng1

  • 1College of Life Sciences, Zhejiang University, Hangzhou 310012, China.

Journal of the American Chemical Society
|September 16, 2024
PubMed
まとめ

研究者らは,重要なタンパク質の改変であるコア・フコシライゼーションを正確に検出するための新しい化学酵素法を開発しました. この進歩は,病気のバイオマーカーを特定し,健康と病気におけるグリカンの役割を理解するための特異性を向上させます.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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