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グライコシライゼーションは,固定された分泌タンパク質の細胞表面輸送を可能にします

J L Guan, C E Machamer, J K Rose

|September 1, 1985

まとめ

膜タンパク質のN-リンクド・グリコシル化により,細胞表面への輸送をシグナルすることができます. この研究は,成長ホルモンにグリコシレーション部位を追加することで,その表面輸送が可能であることを示しています.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
タンパク質の密輸タンパク質の密輸

背景:

ネズミの成長ホルモンを膜に固定するためのハイブリッド遺伝子が作成されました.
このタンパク質は,膀性口腔炎ウイルスのグリコプロテインドメインを膜の固定のために利用します.
タンパク質はゴルギ装置に運ばれますが,細胞表面には届きません.

研究の目的:

細胞表面のタンパク質輸送にN結合型グリコシル化が不可欠であるかどうかを調査する.
膜タンパク質の密輸を誘導するにおけるグリコシレーションの役割を決定する.

主な方法:

In vitro変異遺伝は,変異タンパク質を作成するために使用されました.
特定のアミノ酸の変化により,N-リンクされたグリコシル化のためのコンセンサス配列が導入されました.
野生型および変異型タンパク質の輸送を分析した.

主要な成果:

導入されたグリコシル化部位を持つ変異タンパク質は,グリコシル化に成功しました.
これらのグリコシル化変異タンパク質は,細胞表面に運ばれた.
両方のグリコシル化部位で設計されたタンパク質も,細胞表面に到達した.

結論:

N-リンクされたグリコシレーションは,細胞表面のタンパク質輸送のシグナルとして機能しているようです.
この発見は,タンパク質の密輸経路を制御するメカニズムについての洞察を提供します.

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