MAYEXは,爬虫類のX染色体投与補償のために採用された古い長いノンコーディングRNAです
Mariela Tenorio1, Samantha Cruz-Ruiz2, Sergio Encarnación-Guevara1
1Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México (UNAM), CP62210 Cuernavaca, México.
まとめ
研究者らは緑色のアノールでMAYEXという長いノンコーディングRNA (lncRNA) を発見した. この古代の lncRNAは 性染色体発現のバランスをとり 爬虫類の新種の投与量補給メカニズムを表しています
科学分野:
- 遺伝学とゲノミクス
- エピジェネティクス
- 進化生物学
背景:
- 長い非コーディングRNA (lncRNAs) は,特に性染色体における遺伝子発現の調節に不可欠である.
- 投与補償メカニズムは,様々な種の雄と雌の性染色体発現を均等にします.
- XIST,RSX,roX2のような既知のlncRNAは,哺乳類,有袋類,ドロソフィラのX染色体調節に関与している.
研究 の 目的:
- グリーン・アノール (Anolis carolinensis) の投与補償メカニズムにおける lncRNA の役割を調査する.
- 爬虫類の性染色体調節に関与する新しい lncRNA を特定する.
主な方法:
- lncRNAを特定するための生物情報分析
- 発現パターンを決定する RNA 配列解析
- 染色体免疫降水 (ChIP) で表遺伝的変化を評価する.
- 3Dゲノムコンファメーション技術でロカスループを研究する.
主要な成果:
- アノリス・カロリネンシス (Anolis carolinensis) で 8900万年前に遡る,男性特有の発現を持つ古代の lncRNA,MAYEXの発見
- MAYEXはH4K16acの表遺伝子マークと関連しています.
- MAYEXは X染色体全体にそのロクスをループし,遺伝子の発現を高めます.
結論:
- MAYEXは,投与量補償メカニズムに関与している最初の爬虫類のlncRNAです.
- この発見は,性染色体における lncRNA媒介による遺伝子発現調節のための新しい進化の経路を示しています.
- MAYEXは,爬虫類の性染色体発現のバランスをとる上で重要な役割を果たします.
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