関連する実験動画
Updated: Jun 23, 2026

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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
2つのLyt-2ポリペプチドは,mRNAの代替スプライシングパターンによって1つの遺伝子から発生します
Cell
|November 1, 1985
まとめ
Tリンパ球マーカーであるLyt-2分子は,単一の遺伝子によってコードされています. 代替的なmRNAスプライシングは,サイトプラズマ尾の長さによって異なる2つのLyt-2ポリペプチド鎖を生成します.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- Lyt-2/3分子はTリンパ球のグリコタンパク質である.
- 細胞毒性/抑制性T細胞のマーカーと考えられています.
- それはMHCクラスIタンパク質の受容体であると仮定されています.
研究 の 目的:
- 人間の Leu-2/T8 タンパク質のマウスホモログを特定するために.
- Lyt-2ポリペプチド鎖の多様性の遺伝的基礎を調査する.
- Lyt-2変種を生成する分子メカニズムを解明する.
主な方法:
- マウスのcDNAとヒトの Leu-2/T8 cDNAプローブを用いたゲノムクローンの分離.
- 遺伝子ホモロジーを確認するためのトランスフェクション実験.
- Lyt-2変種をコードするcDNAクローンの核酸配列解析.
主要な成果:
- Leu-2/T8のマウスホモローグは,Lyt-3ではなく,Lyt-2であると特定されました.
- 一つの遺伝子は,2つのLyt-2ポリペプチド鎖をコードする.
- 代替的なmRNAスプライシングは,細胞プラズマの尾の長さの変化につながります.
結論:
- Lyt-2分子は,代替 mRNA スプライシングを通じて単一の遺伝子から生成されます.
- Lyt-2ポリペプチド鎖の観察された差異は,サイトプラズマの尾の長さの違いによるものです.
- この研究は,Tリンパ球におけるLyt-2発現の遺伝的および分子的基礎を明らかにしています.
関連する概念動画
What is Gene Expression?
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

