関連する実験動画

Updated: Jul 15, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

バクテリオファージのRNAポリメラーゼは,Xenopus 5SのRNA遺伝子転写複合体を通して,それを破壊することなく転写します

A P Wolffe, E Jordan, D D Brown

|February 14, 1986

まとめ

この研究は,活性転写複合体を使用して,Xenopus 5S RNA遺伝子転写の速度向上を示しています. プロカリオットSP6RNAポリメラーゼは,転写複合体が結合している場合でも,遺伝子を転写することができ,それは安定しています.

科学分野:

分子生物学は分子生物学である.
遺伝子転写遺伝子転写
Xenopus laevis 研究研究について

背景:

遺伝子転写の調節を理解することは,分子生物学において極めて重要です.
ゼノパス5SRNA遺伝子は,転写の開始と調節を研究するためのモデルシステムです.

研究の目的:

Xenopus 5S RNA遺伝子の活性転写複合体をプログラムするための条件を記述する.
前編成された転写複合体の,プロカリオットポリメラーゼによる後の転写への影響を調査する.

主な方法:

クセノパス・オオサイトの核抽出物を利用する.
Xenopus 5S RNA遺伝子を活性転写複合体でプログラムする.
SP6RNAポリメラーゼによる転写を遺伝子の両鎖で評価する.

主要な成果:

活性転写複合体による転写速度の向上を達成した.
SP6 RNAポリメラーゼの転写は,完全な転写複合体の存在によって妨げられませんでした.
トランスクリプション複合体は,複数のポリメラーゼ通過を経て,安定して遺伝子と関連づけられていました.

結論:

効率的なXenopus 5S RNA遺伝子転写プログラミングのための確立された条件.
外部ポリメラーゼによる継続的な転写を可能にする,転写複合体の強度を示した.
遺伝子の場所における転写複合体の安定性を強調した.

さらに関連する動画

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

関連する概念動画

Bacterial RNA Polymerase

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

Transcription Elongation Factors

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...

Bacterial Transcription

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:

Transcription Initiation

Transcription Initiation

Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...

Bacterial RNA Polymerase

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...