Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme.

Proceedings of the National Academy of Sciences of the United States of America·2004
Same author

Transcription initiation: imposing specificity by localization.

Essays in biochemistry·2002
Same author

Bacterial two-hybrid analysis of interactions between region 4 of the sigma(70) subunit of RNA polymerase and the transcriptional regulators Rsd from Escherichia coli and AlgQ from Pseudomonas aeruginosa.

Journal of bacteriology·2001
Same author

A chimeric activator of transcription that uses two DNA-binding domains to make simultaneous contact with pairs of recognition sites.

Molecular microbiology·2001
Same author

Towards a minimal motif for artificial transcriptional activators.

Chemistry & biology·2001
Same author

Telomere looping permits gene activation by a downstream UAS in yeast.

Nature·2001
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: May 8, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

ラムダ・レプレッサーとラムダ・クロの,ラムダ・オペレータとの同類の相互作用

A Hochschild, M Ptashne

    Cell
    |March 28, 1986
    PubMed
    まとめ
    この要約は機械生成です。

    ラムダ抑制体とCroタンパク質は,ファグ染色体上の類似のDNA部位と結合する. この研究は,両方のタンパク質の特定のセリンが同じDNA位置に接触し,それらの明確な結合親和性を説明することを明らかにしています.

    さらに関連する動画

    CRISPR-Mediated Reorganization of Chromatin Loop Structure
    09:20

    CRISPR-Mediated Reorganization of Chromatin Loop Structure

    Published on: September 14, 2018

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    関連する実験動画

    Last Updated: May 8, 2026

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
    11:19

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

    Published on: August 21, 2016

    CRISPR-Mediated Reorganization of Chromatin Loop Structure
    09:20

    CRISPR-Mediated Reorganization of Chromatin Loop Structure

    Published on: September 14, 2018

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    科学分野:

    • 分子生物学は分子生物学である.
    • 遺伝学 遺伝学とは
    • バイオケミストリー バイオケミストリー

    背景:

    • ラムダ抑制剤とCroは,ファグ染色体上の特定のDNA配列に結合するタンパク質です.
    • それらは共通の結合部位を共有していますが,異なる結合親和性を表しています.
    • 認識ヘリクに保存されたアミノ酸と,オペレータのサイトに保存された核酸は,それらの相互作用の構造的基礎を示唆しています.

    研究 の 目的:

    • ラムダリプレッサーとCro.の両方の認識アルファヘリクスの位置2に保存されたセリンの役割を調査する.
    • このセリン残留物の特定のDNA接触点を決定するために.
    • RepressorとCroが異なる親和性を有する類似のオペレーターサイトを認識する方法の理解を精錬する.

    主な方法:

    • サイト・ダイレクト・ミュータゲネシスにより,セリン残基が変化する.
    • 結合親和性を測定するためのDNA結合測定法.
    • タンパク質とDNAの相互作用を確認するための構造分析.

    主要な成果:

    • 以前のモデルとは異なり,レプレッサーとCroの両方の認識ヘリクスの位置2にあるセリンは,オペレータDNAの位置4にある同じ保存されたヌクレオチドと接触します.
    • この相互作用は,オペレーターサイトの共有認識を説明します.
    • 他の相互作用の差異は,それらの明確な相対的な親和性を説明する可能性がある.

    結論:

    • 保存されたセリン残基は,ラムダ抑制剤とCro.の両方の特定のDNA認識において重要な役割を果たします.
    • これらのタンパク質が類似したオペレータ配列に差異的に結合するための簡素化されたモデルが提案されています.
    • この発見は,タンパク質とDNAの相互作用と配列認識メカニズムについての理解を深める.