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関連する概念動画

Protein Complex Assembly02:41

Protein Complex Assembly

10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

7.2K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
7.2K
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

4.4K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
4.4K
Improving Translational Accuracy02:07

Improving Translational Accuracy

8.8K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
8.8K
Post-translational Translocation of Proteins to the RER01:27

Post-translational Translocation of Proteins to the RER

5.6K
A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
5.6K
Initiation of Translation02:33

Initiation of Translation

30.7K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
30.7K

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関連する実験動画

Updated: Jun 4, 2025

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

2.4K

同翻訳タンパク質複合体の構造的決定因子

Saurav Mallik1, Johannes Venezian2, Arseniy Lobov1

  • 1Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7600001, Israel.

Cell
|December 21, 2024
PubMed
まとめ
この要約は機械生成です。

合成中にタンパク質が組み合わされる 同翻訳性タンパク質組成は 複雑な構造によって引き起こされます このプロセスには,相互に安定させ,遺伝子発現とプロテオスタシスに影響を与える不安定なサブユニットが含まれます.

キーワード:
アルファフォールドコトランスレーションアセンブリmRNAの局所化タンパク質複合体タンパク質の相互作用タンパク質構造プロテオスタシスリボソームプロファイリング単分子 FISH翻訳規制

さらに関連する動画

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

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Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

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関連する実験動画

Last Updated: Jun 4, 2025

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

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Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

3.9K

科学分野:

  • 分子生物学
  • 構造生物学
  • 遺伝学

背景:

  • タンパク質複合体の組成は 生物学的機能に不可欠です
  • タンパク質合成中に発生する同翻訳組成は,人間の細胞でますます認識されています.
  • コトランスレーションアセンブリに関与する基本的なメカニズムと特定のタンパク質パートナーは,ほとんど不明である.

研究 の 目的:

  • コトランスレーションアセンブリを制御する生物学的基礎を解明する.
  • トランスレーション中に組み立てられるタンパク質のペアを識別する.
  • コトランスレーションアセンブリの予測フレームワークを開発する.

主な方法:

  • タンパク質複合体の構造特性の分析
  • AlphaFold2の予測を使って 構造的なサインを特定する
  • リボソームプロファイリング,ステキオメトリー乱射,および単分子RNA-光 in situハイブリデーション (smFISH) を用いた実験的検証.

主要な成果:

  • コトランスレーションアセンブリは,タンパク質複合体の構造特性によって決定される.
  • コトランスレーションアセンブリに関与するサブユニットは,孤立した状態では不安定であり,相互に安定化します.
  • 構造的シグネチャーとAlphaFold2の予測は,種間のプロテオーム全体のスケールで協同翻訳組成ペアを正確に特定しました.

結論:

  • タンパク質の3次元構造と 翻訳プロセスの間の 根本的なリンクを確立しました
  • 構造が遺伝子発現,mRNAの局所化,およびプロテオスタシスに有意な影響を及ぼしていることを実証した.
  • コトランスレーションアセンブリは 細胞機能に不可欠な構造駆動メカニズムです