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Updated: Jun 3, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

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私たちの間に橋はない:EXAFSと計算は,遠隔の2つの鉄イオンがアルカン単酸化酵素 (AlkB) の活性部位を含むことを確認する

Clorice R Reinhardt1, Juliet A Lee2, Lauren Hendricks2

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

Journal of the American Chemical Society
|January 8, 2025
PubMed
まとめ

アルカン一酸化酵素 (AlkB) 酵素は,独特の鉄結合活性部位を特徴としています. 顕微鏡およびシミュレーション研究では,D190が重要な触媒の役割を果たす橋渡しリガンドの欠如が確認されています.

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