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Updated: Jun 2, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

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基本冷凍画像は,SOS1依存の真空ナトリウム蓄積を明らかにする

Priya Ramakrishna1,2, Francisco M Gámez-Arjona3,4, Etienne Bellani5,6

  • 1Laboratory for Biological Geochemistry, School of Architecture, Civil and Environmental Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. priya.ramakrishna@epfl.ch.

Nature
|January 15, 2025
PubMed
まとめ

植物の塩分耐性を理解することは 収穫量にとって極めて重要です この研究は,ストレス反応の重要なメカニズムであるSOS1/NHX7アンチポーターによって,バキュオールに蓄積することによって,植物がナトリウム (Na+) を管理する方法を明らかにしています.

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Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells11:45

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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